##gff-version 3
##sequence-region BBa_K1740004 1 1642
BBa_K1740004	.	sequence_feature	939	944	.	+	0	ID=annotation2446814;Name=Kpn I
BBa_K1740004	.	sequence_alteration	465	467	.	+	0	ID=annotation2446810;Name=145Lys to 145Asn
BBa_K1740004	.	CDS	227	766	.	+	0	ID=annotation2446809;Name=Dronpa 145N
BBa_K1740004	.	sequence_feature	941	946	.	+	0	ID=annotation2446815;Name=Sal I
BBa_K1740004	.	sequence_feature	951	956	.	+	0	ID=annotation2446816;Name=Sac I
BBa_K1740004	.	CDS	965	1636	.	+	0	ID=annotation2446818;Name=Dronpa 145N
BBa_K1740004	.	sequence_feature	959	964	.	+	0	ID=annotation2446817;Name=BamH I
BBa_K1740004	.	ribosome_entry_site	209	222	.	+	0	ID=annotation2446808;Name=RBS
BBa_K1740004	.	sequence_alteration	1203	1205	.	+	0	ID=annotation2446819;Name=145Lys to 145Asn
BBa_K1740004	.	engineered_region	1	200	.	+	0	ID=annotation2446807;Name=pLac
BBa_K1740004	.	sequence_feature	930	935	.	+	0	ID=annotation2446813;Name=Nde I
BBa_K1740004	.	sequence_feature	917	922	.	+	0	ID=annotation2446812;Name=Hind III
BBa_K1740004	.	sequence_feature	911	916	.	+	0	ID=annotation2446811;Name=Xho I
BBa_K1740004	.	stop_codon	1637	1642	.	+	0	ID=annotation2446820;Name=Double stop codon
>BBa_K1740004
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtcagtcat
caagcctgacatgaaaattaaattgcgaatggaaggtgctgtaaacgggcacccgttcgctatagaaggggtagggttg
ggcaagcccttcgaaggaaagcagagtatggatcttaaagttaaagagggtggaccgttaccatttgcctatgacattc
tcactacagtattctgctatggcaaccgtgttttcgcaaagtatccagaaaatatcgttgactacttcaaacagtcgtt
tcccgagggctattcgtgggagcgctctatgaattacgaggacggtggtatctgtaatgccaccaacgatattaccctg
gacggtgactgttatatttatgaaatccgctttgatggtgtgaattttcctgcaaatggtccggtgatgcaaaaacgga
ccgttaaatgggagccgagcacggaaaacctatatgtccgcgatggcgtgctgaaaggcgatgtgaacatggcgctgag
cttagaaggcggcggccattaccgttgcgattttaaaacgacctacaaagcgaaaaaagtcgtccaactgccggattac
cactttgtggatcatcatatcgaaattaaatcccatgataaagattacagcaacgtgaacctgcacgaacatgccgaag
cgcacagtgaactgccgcgtcaggcgaaactcgagtaataactcgagaagctttggccacatatgggtaccgtcgacga
gctccccgggggatccatgtcagtcatcaagcctgacatgaaaattaaattgcgaatggaaggtgctgtaaacgggcac
ccgttcgctatagaaggggtagggttgggcaagcccttcgaaggaaagcagagtatggatcttaaagttaaagagggtg
gaccgttaccatttgcctatgacattctcactacagtattctgctatggcaaccgtgttttcgcaaagtatccagaaaa
tatcgttgactacttcaaacagtcgtttcccgagggctattcgtgggagcgctctatgaattacgaggacggtggtatc
tgtaatgccaccaacgatattaccctggacggtgactgttatatttatgaaatccgctttgatggtgtgaattttcctg
caaatggtccggtgatgcaaaaacggaccgttaaatgggagccgagcacggaaaacctatatgtccgcgatggcgtgct
gaaaggcgatgtgaacatggcgctgagcttagaaggcggcggccattaccgttgcgattttaaaacgacctacaaagcg
aaaaaagtcgtccaactgccggattaccactttgtggatcatcatatcgaaattaaatcccatgataaagattacagca
acgtgaacctgcacgaacatgccgaagcgcacagtgaactgccgcgtcaggcgaaataataa