BBa_K174001 1 BBa_K174001 Arabinose inducible system 2009-09-25T11:00:00Z 2015-05-08T01:10:58Z Wild-type Bacillus subtilis 168 strain. 200 bp long bindingsite_promoter_bindingsite_RBS is amplified by PCR with dangling end primers with EcoRI-NotI-XbaI restriction site at 5??? and SpeI at 3??? and inserted into a Biobrick compatible vector. The sequence is taken from wildtype Bacillus subtilis Comprised of araR binding sites and araE promoter. Regulates the expression of downstream genes with arabinose. false false _277_ 0 3942 9 It's complicated false Forward primer used: GATCTG-GAATTCGCGGCCGCTTCTAGAG-CACAGCGTTCTTTGTAAG (Clamp sequence - Standard Biobrick prefix - first 18 base from the Biobrick) Reverse primer used: TGTGAC-ACTAGTA-GCCCTCCCGAATGTTGAG (Clamp sequence - SpeI site - last 18 base from the Biobrick) false The Newcastle 2009 iGEM team annotation2027218 1 araR binding site range2027218 1 154 178 annotation2027217 1 sigA promoter range2027217 1 80 123 annotation2027219 1 rbs range2027219 1 208 214 annotation2027216 1 araR binding site range2027216 1 32 51 BBa_K174001_sequence 1 cgcgtattttggtaacatatccattcctccaaaatgtatacggacaaatttcagtatatcacagcgttctttgtaagaaaacattgacagaaaatgcaaacaagattattctatatttgtacgtactaattaaatgtaattttcgttaaattttaatataagtacgtacaattgaaggtttaaatgaaaacgctttactcaacattcgggagggc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z