BBa_K174000
1
BBa_K174000
SspB proteolysis chaperone
2009-09-25T11:00:00Z
2015-05-08T01:10:58Z
498 bp long sspB CDS is amplified by PCR with dangling end primers with EcoRI-NotI-XbaI restriction site at 5??? and SpeI at 3??? and inserted into a Biobrick compatible vector. The sequence is taken from E. coli strain DH5 alpha. Genbank accession number for E. coli MG1655 strain is NC_000913.2
SspB protein targets proteins tagged with modified degradation tag, ssrA, and deliver them to ClpXP for proteolysis. Modified ssrA tags are fused onto the 3??? end of a gene.
false
false
_277_
0
3942
9
It's complicated
false
Forward primer used: GATCTG-GAATTCGCGGCCGCTTCTAG-ATGGATTTGTCACAGCTAAC
(Clamp sequence - Standard Biobrick prefix - first 20 base from the Biobrick)
Reverse primer used: TGTGAC-ACTAGTA-TTACTTCACAACGCGTAATGC
(Clamp sequence - SpeI site - last 21 base from the Biobrick)
false
The Newcastle 2009 iGEM team
annotation2027215
1
sspB
range2027215
1
1
498
BBa_K174002
1
BBa_K174002
Arabinose controlled protein degradation device
2009-09-25T11:00:00Z
2015-05-08T01:10:58Z
Comprised of other Biobricks
The purpose of this system is to control the expression of sspB, the degradation controller. The system is inducible by arabinose, therefore we can alter the arabinose levels to regulate the degradation of proteins with sspB degradation tag
false
false
_277_
0
3942
9
It's complicated
true
sspB Biobrick is inserted after araR promoter+rbs system
false
The Newcastle 2009 iGEM team
component2027244
1
BBa_K174001
component2027246
1
BBa_K174000
annotation2027244
1
BBa_K174001
range2027244
1
1
215
annotation2027246
1
BBa_K174000
range2027246
1
222
719
BBa_K174001
1
BBa_K174001
Arabinose inducible system
2009-09-25T11:00:00Z
2015-05-08T01:10:58Z
Wild-type Bacillus subtilis 168 strain. 200 bp long bindingsite_promoter_bindingsite_RBS is amplified by PCR with dangling end primers with EcoRI-NotI-XbaI restriction site at 5??? and SpeI at 3??? and inserted into a Biobrick compatible vector. The sequence is taken from wildtype Bacillus subtilis
Comprised of araR binding sites and araE promoter. Regulates the expression of downstream genes with arabinose.
false
false
_277_
0
3942
9
It's complicated
false
Forward primer used: GATCTG-GAATTCGCGGCCGCTTCTAGAG-CACAGCGTTCTTTGTAAG
(Clamp sequence - Standard Biobrick prefix - first 18 base from the Biobrick)
Reverse primer used: TGTGAC-ACTAGTA-GCCCTCCCGAATGTTGAG
(Clamp sequence - SpeI site - last 18 base from the Biobrick)
false
The Newcastle 2009 iGEM team
annotation2027218
1
araR binding site
range2027218
1
154
178
annotation2027216
1
araR binding site
range2027216
1
32
51
annotation2027217
1
sigA promoter
range2027217
1
80
123
annotation2027219
1
rbs
range2027219
1
208
214
BBa_K174001_sequence
1
cgcgtattttggtaacatatccattcctccaaaatgtatacggacaaatttcagtatatcacagcgttctttgtaagaaaacattgacagaaaatgcaaacaagattattctatatttgtacgtactaattaaatgtaattttcgttaaattttaatataagtacgtacaattgaaggtttaaatgaaaacgctttactcaacattcgggagggc
BBa_K174000_sequence
1
atggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggcgcaggcacgatgtttgagcctgaagctgcctacgatgaagataccagcatcatgaatgatgaagaggcatcggcagacaacgaaaccgttatgtcggttattgatggcgacaagccagatcacgatgatgacactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaa
BBa_K174002_sequence
1
cgcgtattttggtaacatatccattcctccaaaatgtatacggacaaatttcagtatatcacagcgttctttgtaagaaaacattgacagaaaatgcaaacaagattattctatatttgtacgtactaattaaatgtaattttcgttaaattttaatataagtacgtacaattgaaggtttaaatgaaaacgctttactcaacattcgggagggctactagatggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggcgcaggcacgatgtttgagcctgaagctgcctacgatgaagataccagcatcatgaatgatgaagaggcatcggcagacaacgaaaccgttatgtcggttattgatggcgacaagccagatcacgatgatgacactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z