LOCUS       BBa_K174003             1928 bp    DNA     linear       15-Jun-2024
DEFINITION  Heritable, Tunable, Stochastic Switch
ACCESSION   BBa_K174003
VERSION     BBa_K174003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..95
                     /label=BBa_B0014
     misc_feature    96..776
                     /label=BBa_E1010 (reverse)
     RBS             777..793
                     /label=RBS from pmutin4_lacZ (reverse)
     misc_feature    794..799
                     /label=AccIII Cut Site
     misc_feature    883..896
                     /label=LacI binding site (oid)
     promoter        800..905
                     /label=promoter from pmutin4_lacZ
     misc_feature    906..911
                     /label=BalI Cut Site
     misc_feature    912..937
                     /label=BBa_J44000
     misc_feature    938..1034
                     /label=BBa_K143012 sigA promoter (pveg-reverse)
     RBS             1035..1051
                     /label=RBS from pmutin4_lacZ
     misc_feature    1052..1621
                     /label=BBa_J31001 Hin CDS
     misc_feature    1622..1666
                     /label=ssrA ALGG degradation tag
     misc_feature    1667..1669
                     /label=Hin stop codon
     misc_feature    1670..1764
                     /label=BBa_B0014 Double Terminator
     misc_feature    1765..1790
                     /label=BBa_J44000 HixC
     misc_feature    1791..1796
                     /label=NaeI Cut Site
     promoter        1797..1922
                     /label=xylA promoter (reverse)
     misc_feature    1923..1928
                     /label=NruI Cut Site
ORIGIN
        1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
       61 aaagccagat tattaatccg gcttttttat tatttttatt aagcaccggt ggagtgacga
      121 ccttcagcac gttcgtactg ttcaacgatg gtgtagtctt cgttgtggga ggtgatgtcc
      181 agtttgatgt cggttttgta agcacccggc agctgaaccg gttttttagc catgtaggtg
      241 gttttaactt cagcgtcgta gtgaccaccg tctttcagtt tcagacgcat tttgatttca
      301 cctttcagag caccgtcttc cgggtacata cgttcggtgg aagcttccca acccatggtt
      361 tttttctgca taaccggacc gtcggacggg aagttggtac cacgcagttt aactttgtag
      421 atgaactcac cgtcttgcag ggaggagtcc tgggtaacgg taacaacacc accgtcttcg
      481 aagttcataa cacgttccca tttgaaacct tccgggaagg acagtttcag gtagtccggg
      541 atgtcagccg ggtgtttaac gtaagctttg gaaccgtact ggaactgcgg ggacaggatg
      601 tcccaagcga acggcagcgg accacctttg gtaactttca gtttagcggt ctgggtacct
      661 tcgtacggac gaccttcacc ttcaccttcg atttcgaact cgtgaccgtt aacggaacct
      721 tccatacgaa ctttgaaacg catgaactct ttgataacgt cttcggagga agccatagta
      781 gttcaccacc ttttccggaa gaacaacctc tgctaaaatt cctgaaaaat tttgcaaaaa
      841 gttgttgact ttatctacaa ggtgtggcat aatgtgtgga attgtgagcg ctcacaatta
      901 agctttggcc attatcaaaa accatggttt ttgataaaca tttattgtac aacacgagcc
      961 catttttgtc aaataaaatt taaattatat caacgttaat aagacgttgt caataaaatt
     1021 attttgacaa aattaaaggt ggtgaactac tatggctact attgggtata ttcgggtgtc
     1081 aacaattgac caaaatatcg atttacagcg taatgcgctt accagtgcaa attgtgaccg
     1141 catttttgag gaccgtatca gtggcaagat tgcaaaccgc cccggcctga aacgagcgtt
     1201 aaagtatgta aataaaggcg atactcttgt cgtctggaaa ttagacagac tgggccgcag
     1261 cgtgaaaaac ctggtggcgt taatatcaga attacatgaa cgtggagctc acttccattc
     1321 tttaaccgat agtattgata ccagtagcgc gatggggcga ttcttttttc atgtaatgtc
     1381 agcactggcc gagatggagc gagaattaat tgtcgagcga acccttgccg gactggctgc
     1441 cgccagagcg caaggacgac tgggagggcg ccctcgggcg atcaacagac atgaacagga
     1501 acagattagt cggctattag agaaaggcca tcctcggcag caactagcta ttatttttgg
     1561 tattggcgta tctaccttat acagatattt tccggcaagc cgtataaaaa aacgaatgaa
     1621 tgctgcaaat gatgaaaatt atagcgaaaa ttatgctctt ggcggctaat cacactggct
     1681 caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt
     1741 attaatccgg cttttttatt atttttatca aaaaccatgg tttttgataa gccggcttgt
     1801 catttccccc tttgatttaa gtgaacaagt ttatccatca actatcttaa ttgagttagt
     1861 ttgtttatcc aataaactaa ctttatctca tcatatacaa aataaatgtt tatttcaatg
     1921 tttcgcga
//