##gff-version 3
##sequence-region BBa_K174003 1 1928
BBa_K174003	.	ribosome_entry_site	1035	1051	.	+	0	ID=annotation2028013;Name=RBS from pmutin4_lacZ
BBa_K174003	.	sequence_feature	1791	1796	.	+	0	ID=annotation2028039;Name=NaeI Cut Site
BBa_K174003	.	engineered_region	1670	1764	.	+	0	ID=annotation2028037;Name=BBa_B0014 Double Terminator
BBa_K174003	.	sequence_feature	794	799	.	+	0	ID=annotation2028003;Name=AccIII Cut Site
BBa_K174003	.	non_covalent_binding_site	883	896	.	+	0	ID=annotation2028005;Name=LacI binding site (oid)
BBa_K174003	.	ribosome_entry_site	777	793	.	+	0	ID=annotation2028002;Name=RBS from pmutin4_lacZ (reverse)
BBa_K174003	.	non_covalent_binding_site	1622	1666	.	+	0	ID=annotation2028035;Name=ssrA ALGG degradation tag
BBa_K174003	.	engineered_region	912	937	.	+	0	ID=annotation2028010;Name=BBa_J44000
BBa_K174003	.	promoter	1797	1922	.	+	0	ID=annotation2028041;Name=xylA promoter (reverse)
BBa_K174003	.	engineered_region	1	95	.	+	0	ID=annotation2028000;Name=BBa_B0014
BBa_K174003	.	sequence_feature	906	911	.	+	0	ID=annotation2028009;Name=BalI Cut Site
BBa_K174003	.	engineered_region	938	1034	.	+	0	ID=annotation2028012;Name=BBa_K143012 sigA promoter (pveg-reverse)
BBa_K174003	.	stop_codon	1667	1669	.	+	0	ID=annotation2028036;Name=Hin stop codon
BBa_K174003	.	promoter	800	905	.	+	0	ID=annotation2028006;Name=promoter from pmutin4_lacZ
BBa_K174003	.	engineered_region	1765	1790	.	+	0	ID=annotation2028038;Name=BBa_J44000 HixC
BBa_K174003	.	engineered_region	96	776	.	+	0	ID=annotation2028001;Name=BBa_E1010 (reverse)
BBa_K174003	.	sequence_feature	1923	1928	.	+	0	ID=annotation2028043;Name=NruI Cut Site
BBa_K174003	.	engineered_region	1052	1621	.	+	0	ID=annotation2028034;Name=BBa_J31001 Hin CDS
>BBa_K174003
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatcc
ggcttttttattatttttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtc
ttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtag
gtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgt
cttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagtt
ggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccg
tcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggt
gtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggt
aactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgtta
acggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatagtagttcaccacc
ttttccggaagaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggca
taatgtgtggaattgtgagcgctcacaattaagctttggccattatcaaaaaccatggtttttgataaacatttattgt
acaacacgagcccatttttgtcaaataaaatttaaattatatcaacgttaataagacgttgtcaataaaattattttga
caaaattaaaggtggtgaactactatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttac
agcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccgg
cctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtg
aaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgatacca
gtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaac
ccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaa
cagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttat
acagatattttccggcaagccgtataaaaaaacgaatgaatgctgcaaatgatgaaaattatagcgaaaattatgctct
tggcggctaatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccaga
ttattaatccggcttttttattatttttatcaaaaaccatggtttttgataagccggcttgtcatttccccctttgatt
taagtgaacaagtttatccatcaactatcttaattgagttagtttgtttatccaataaactaactttatctcatcatat
acaaaataaatgtttatttcaatgtttcgcga