BBa_K174003
1
BBa_K174003
Heritable, Tunable, Stochastic Switch
2009-10-03T11:00:00Z
2015-05-08T01:10:58Z
Synthesised
This tunable switch is biased heads or tails. It can be tuned to control cell differention and fate stochastically.
false
false
_277_
0
3942
9
It's complicated
true
It can be tuned by IPTG and Xylose however if one wishes to control the switch with something else, the control regions can easily be replaced since they are surrounded with restriction sites.
false
The Newcastle 2009 iGEM team
annotation2028013
1
RBS from pmutin4_lacZ
range2028013
1
1035
1051
annotation2028002
1
RBS from pmutin4_lacZ (reverse)
range2028002
1
777
793
annotation2028006
1
promoter from pmutin4_lacZ
range2028006
1
800
905
annotation2028038
1
BBa_J44000 HixC
range2028038
1
1765
1790
annotation2028039
1
NaeI Cut Site
range2028039
1
1791
1796
annotation2028005
1
LacI binding site (oid)
range2028005
1
883
896
annotation2028035
1
ssrA ALGG degradation tag
range2028035
1
1622
1666
annotation2028000
1
BBa_B0014
range2028000
1
1
95
annotation2028036
1
Hin stop codon
range2028036
1
1667
1669
annotation2028041
1
xylA promoter (reverse)
range2028041
1
1797
1922
annotation2028037
1
BBa_B0014 Double Terminator
range2028037
1
1670
1764
annotation2028009
1
BalI Cut Site
range2028009
1
906
911
annotation2028001
1
BBa_E1010 (reverse)
range2028001
1
96
776
annotation2028010
1
BBa_J44000
range2028010
1
912
937
annotation2028034
1
BBa_J31001 Hin CDS
range2028034
1
1052
1621
annotation2028043
1
NruI Cut Site
range2028043
1
1923
1928
annotation2028003
1
AccIII Cut Site
range2028003
1
794
799
annotation2028012
1
BBa_K143012 sigA promoter (pveg-reverse)
range2028012
1
938
1034
BBa_K174003_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatagtagttcaccaccttttccggaagaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcgctcacaattaagctttggccattatcaaaaaccatggtttttgataaacatttattgtacaacacgagcccatttttgtcaaataaaatttaaattatatcaacgttaataagacgttgtcaataaaattattttgacaaaattaaaggtggtgaactactatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaatgctgcaaatgatgaaaattatagcgaaaattatgctcttggcggctaatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttttatcaaaaaccatggtttttgataagccggcttgtcatttccccctttgatttaagtgaacaagtttatccatcaactatcttaattgagttagtttgtttatccaataaactaactttatctcatcatatacaaaataaatgtttatttcaatgtttcgcga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z