BBa_K174005
1
BBa_K174005
xylA promoter
2009-10-09T11:00:00Z
2015-05-08T01:10:58Z
This part is based on pX integration vector which has xlyA promoter with xlyR operator sites.
This promoter can be induced by xylose and it is regulated by LacI. XylR is normally expressed i B. subtilis and represses the promoter. Addition of xylose will derepress XlyR activity on the promoter.
Biobrick BBa_K143014 is also xylose inducable promoter. They have slightly different operator sites and -10 sequence is one base different. Hence this promoter may have different promoter strength and regulation by XlyR.
It should be noted that glucose may act as an anti-inducer for xlyR triggering catabolite repression.
false
false
_277_
0
3942
9
Not in stock
false
The sequence from the beginning of xlyA promoter to the start codon just before SpeI site was used to build the part.
false
The Newcastle 2009 iGEM team
annotation2033097
1
XylR operator
range2033097
1
43
52
annotation2033096
1
-35
range2033096
1
5
10
annotation2033095
1
-10
range2033095
1
28
33
annotation2033098
1
XylR Operator
range2033098
1
58
67
BBa_K174005_sequence
1
aacattgaaataaacatttattttgtatatgatgagataaagttagtttattggataaacaaactaactcaattaagatagttgatggataaacttgttcacttaaatcaaagggggaaatgacaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z