BBa_K174007 1 BBa_K174007 Degradation controller with integration site 2009-10-09T11:00:00Z 2015-05-08T01:10:58Z Ligated manually When inserted into a ''Bacillus subtilis'' integration vector, it will provide a single crossover site with arabinose controlled sspB expression. This device will be integrated onto the sacA gene on the chromosome false false _277_ 0 3942 9 Not in stock false To test the integration, pmutin4 integration vector is selected. However after the integration pspac is left on the right side, free to drive the expression of downstream genes. To remove the effect of pspac promoter of pmutin4 vector, the device with ligation of sac integration site, araR repressible promoter and sspB CDS was amplified with PacI from one end. Hence by cutting pmutin4 with PacI, pspac promoter can be removed. For the other end, sacII was selected since it is compatible with PacI. PacI is just on the left side of Terminator0 on pmutin4, it will also be removed leaving Terminator 1 and Terminator 3 on the vector. false The Newcastle 2009 iGEM team component2033150 1 BBa_K174000 component2033148 1 BBa_K174001 component2033143 1 BBa_K174006 annotation2033148 1 BBa_K174001 range2033148 1 375 589 annotation2033143 1 BBa_K174006 range2033143 1 1 366 annotation2033150 1 BBa_K174000 range2033150 1 596 1093 BBa_K174000 1 BBa_K174000 SspB proteolysis chaperone 2009-09-25T11:00:00Z 2015-05-08T01:10:58Z 498 bp long sspB CDS is amplified by PCR with dangling end primers with EcoRI-NotI-XbaI restriction site at 5??? and SpeI at 3??? and inserted into a Biobrick compatible vector. The sequence is taken from E. coli strain DH5 alpha. Genbank accession number for E. coli MG1655 strain is NC_000913.2 SspB protein targets proteins tagged with modified degradation tag, ssrA, and deliver them to ClpXP for proteolysis. Modified ssrA tags are fused onto the 3??? end of a gene. false false _277_ 0 3942 9 It's complicated false Forward primer used: GATCTG-GAATTCGCGGCCGCTTCTAG-ATGGATTTGTCACAGCTAAC (Clamp sequence - Standard Biobrick prefix - first 20 base from the Biobrick) Reverse primer used: TGTGAC-ACTAGTA-TTACTTCACAACGCGTAATGC (Clamp sequence - SpeI site - last 21 base from the Biobrick) false The Newcastle 2009 iGEM team annotation2027215 1 sspB range2027215 1 1 498 BBa_K174001 1 BBa_K174001 Arabinose inducible system 2009-09-25T11:00:00Z 2015-05-08T01:10:58Z Wild-type Bacillus subtilis 168 strain. 200 bp long bindingsite_promoter_bindingsite_RBS is amplified by PCR with dangling end primers with EcoRI-NotI-XbaI restriction site at 5??? and SpeI at 3??? and inserted into a Biobrick compatible vector. The sequence is taken from wildtype Bacillus subtilis Comprised of araR binding sites and araE promoter. Regulates the expression of downstream genes with arabinose. false false _277_ 0 3942 9 It's complicated false Forward primer used: GATCTG-GAATTCGCGGCCGCTTCTAGAG-CACAGCGTTCTTTGTAAG (Clamp sequence - Standard Biobrick prefix - first 18 base from the Biobrick) Reverse primer used: TGTGAC-ACTAGTA-GCCCTCCCGAATGTTGAG (Clamp sequence - SpeI site - last 18 base from the Biobrick) false The Newcastle 2009 iGEM team annotation2027217 1 sigA promoter range2027217 1 80 123 annotation2027219 1 rbs range2027219 1 208 214 annotation2027216 1 araR binding site range2027216 1 32 51 annotation2027218 1 araR binding site range2027218 1 154 178 BBa_K174006 1 BBa_K174006 Sac single crossover site for Bacillus subtilis 2009-10-09T11:00:00Z 2015-05-08T01:10:58Z The sequence is taken from 'B. subtilis' 168' sacA gene. When inserted into a 'Bacillus subtilis' integration vector, it provides a single crossover copying the whole plasmid into the bacterial chromosome. Hence this integration biobrick can be ligated with the biobrick that is wanted to be transferred into Bacillus subtilis and inserted into an integration vector such as pmutin4. false false _277_ 0 3942 9 Not in stock false The sequence selected does not contain any restriction site specific to Biobrick standards false The Newcastle 2009 iGEM team BBa_K174001_sequence 1 cgcgtattttggtaacatatccattcctccaaaatgtatacggacaaatttcagtatatcacagcgttctttgtaagaaaacattgacagaaaatgcaaacaagattattctatatttgtacgtactaattaaatgtaattttcgttaaattttaatataagtacgtacaattgaaggtttaaatgaaaacgctttactcaacattcgggagggc BBa_K174000_sequence 1 atggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggcgcaggcacgatgtttgagcctgaagctgcctacgatgaagataccagcatcatgaatgatgaagaggcatcggcagacaacgaaaccgttatgtcggttattgatggcgacaagccagatcacgatgatgacactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaa BBa_K174006_sequence 1 ttctcatttctgcgcatggcttttagctcaggcagcggctgctgaatcagcttctgtcctgaaagcgtcagctgtctcggcagcgtcatgcagtgaatccagtggcagtcaatggtcggatgggacccttcatcctgatcaggcaccgccatccatgcaaataaaatccgccttccctgatcgtcttcaagtgtttgcggcgcgtaaaaatcaaaaccttgatcaagctccgtaaattcaccatgcttcagttcaggcttgttataatcgaggcggccgacaaaataacctgattgatatacgttctgataacggaaaccgtcagcctcaagcccttgaggcgaaacaatcagcacatccgatcct BBa_K174007_sequence 1 ttctcatttctgcgcatggcttttagctcaggcagcggctgctgaatcagcttctgtcctgaaagcgtcagctgtctcggcagcgtcatgcagtgaatccagtggcagtcaatggtcggatgggacccttcatcctgatcaggcaccgccatccatgcaaataaaatccgccttccctgatcgtcttcaagtgtttgcggcgcgtaaaaatcaaaaccttgatcaagctccgtaaattcaccatgcttcagttcaggcttgttataatcgaggcggccgacaaaataacctgattgatatacgttctgataacggaaaccgtcagcctcaagcccttgaggcgaaacaatcagcacatccgatccttactagagcgcgtattttggtaacatatccattcctccaaaatgtatacggacaaatttcagtatatcacagcgttctttgtaagaaaacattgacagaaaatgcaaacaagattattctatatttgtacgtactaattaaatgtaattttcgttaaattttaatataagtacgtacaattgaaggtttaaatgaaaacgctttactcaacattcgggagggctactagatggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggcgcaggcacgatgtttgagcctgaagctgcctacgatgaagataccagcatcatgaatgatgaagaggcatcggcagacaacgaaaccgttatgtcggttattgatggcgacaagccagatcacgatgatgacactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z