BBa_K174014 1 BBa_K174014 cwlJ + sleB, B. subtilis germination genes 2009-10-13T11:00:00Z 2015-05-08T01:10:58Z B. subtilis Both CwlJ and SleB are germination specific cortex hydrolases. Although SleB has a major effect for the germination, cwlJ's effect is minor. However bacillus subtilis with double mutation without cwlJ and sleB were not able germinate again (2). To control the germination rate tightly, cwlJ and sleB genes are combined to make this device. false false _277_ 0 3942 9 It's complicated false For tight regulation of germination, we decided to combine cwlJ and sleB. false The Newcastle 2009 iGEM team component2039845 1 BBa_K174012 component2039842 1 BBa_K174013 annotation2039842 1 BBa_K174013 range2039842 1 1 441 annotation2039845 1 BBa_K174012 range2039845 1 450 1381 BBa_K174013 1 BBa_K174013 cwlJ, Bacillus subtilis germination gene with RBS 2009-10-13T11:00:00Z 2015-05-08T01:10:58Z ''B. subtilis'' ''cwlJ'' as a cell wall hydrolase gene and it effects the germination of spores. false false _277_ 0 3942 9 It's complicated false It has been added as a part with its own natural RBS. false The Newcastle 2009 iGEM team annotation2039829 1 cwlJ range2039829 1 13 441 annotation2039828 1 RBS range2039828 1 1 8 BBa_K174012 1 BBa_K174012 sleB, Bacillus subtilis germination gene with RBS 2009-10-13T11:00:00Z 2015-05-08T01:10:58Z ''B. subtilis'' ''sleB'' encodes for a spore cortex-lytic enzyme. This enzyme responds germination triggered by L-alanine which is the most universal germinant for spores. It has been shown that B. subtilis mutants with inactivated ''sleB'' gene were not able to germinate (1). Hence by regulating this gene in ''sleB'' mutant ''B. subtilis'' cells, germination rate can be contolled. false false _277_ 0 3942 9 It's complicated false It has been added as a part with its own natural RBS. false The Newcastle 2009 iGEM team annotation2039826 1 RBS range2039826 1 1 5 annotation2039827 1 SleB CDS range2039827 1 15 932 BBa_K174014_sequence 1 ggaggtgacgtgatggcggtcgtgagagcaacgagtgcggatgtcgatttgatggcaaggctgctcagagcggaagcggaaggcgaaggcaagcaggggatgctgcttgtcggcaacgttggaattaatcggctgcgggcgaattgctcagattttaaaggcctccgcaccatcaggcagatgatttatcagccacacgcgtttgaggctgtgactcatggatatttttatcaaagggcgcgagatagcgagcgtgcccttgcacgcggctcgattaatggtgaaaggcgctggcctgcaaaatttagtttatggtacttcaggccgcagggggactgtccagcccagtggtataaccagccgtttgtggccagatttaagtcacactgcttttatcagccgacggcggagacgtgtgaaaatgtatataacacattttagtactagagggaggcttgaaaagatgaagtccaaaggatcgattatggcatgtctcatccttttttcctttacaataacgacgtttattaatactgaaacgatctctgccttttcgaatcaggtcattcaaagaggggcaacaggggatgatgtggtcgagcttcaggcgcgtcttcaatacaacggatattataacggaaaaattgacggggtttatggatgggggacgtactgggcagttcgaaattttcaggatcaattcgggttaaaagaggttgacggccttgtaggagctaaaacaaagcaaaccttaatatgtaaatcaaaatactatcgtgaatatgtcatggaacagctcaataaagggaatacattcacgcattacggaaaaattccgctaaagtatcagacgaaaccatcaaaagcagcaacacaaaaggcaagacaacaagcagaagcacggcagaaacagcctgcggaaaaaacaacgcagaagcctaaagcgaatgcgaataaacagcaaaacaatacaccagcaaaagcaagaaaacaggatgcggtagcagcgaacatgcctggtggattttccaacaacgatatcaggctgcttgctcaagcggtttatggcgaagcccggggcgagccgtacgaggggcaggttgctattgcagcagtcattttaaaccgtttgaacagcccgttatttccaaattcagtagcgggggttatttttgagccgcttgccttcacagcagtagccgacggacaaatttacatgcagccgaatgaaacggcacgagaagcagtgctggatgccatcaatggctgggacccatcagaggaagcactttactactttaatccggatacggctacaagtccgtggatttgggggcgtccgcagattaaaagaatcggtaaacacattttctgtgagtag BBa_K174012_sequence 1 ggaggcttgaaaagatgaagtccaaaggatcgattatggcatgtctcatccttttttcctttacaataacgacgtttattaatactgaaacgatctctgccttttcgaatcaggtcattcaaagaggggcaacaggggatgatgtggtcgagcttcaggcgcgtcttcaatacaacggatattataacggaaaaattgacggggtttatggatgggggacgtactgggcagttcgaaattttcaggatcaattcgggttaaaagaggttgacggccttgtaggagctaaaacaaagcaaaccttaatatgtaaatcaaaatactatcgtgaatatgtcatggaacagctcaataaagggaatacattcacgcattacggaaaaattccgctaaagtatcagacgaaaccatcaaaagcagcaacacaaaaggcaagacaacaagcagaagcacggcagaaacagcctgcggaaaaaacaacgcagaagcctaaagcgaatgcgaataaacagcaaaacaatacaccagcaaaagcaagaaaacaggatgcggtagcagcgaacatgcctggtggattttccaacaacgatatcaggctgcttgctcaagcggtttatggcgaagcccggggcgagccgtacgaggggcaggttgctattgcagcagtcattttaaaccgtttgaacagcccgttatttccaaattcagtagcgggggttatttttgagccgcttgccttcacagcagtagccgacggacaaatttacatgcagccgaatgaaacggcacgagaagcagtgctggatgccatcaatggctgggacccatcagaggaagcactttactactttaatccggatacggctacaagtccgtggatttgggggcgtccgcagattaaaagaatcggtaaacacattttctgtgagtag BBa_K174013_sequence 1 ggaggtgacgtgatggcggtcgtgagagcaacgagtgcggatgtcgatttgatggcaaggctgctcagagcggaagcggaaggcgaaggcaagcaggggatgctgcttgtcggcaacgttggaattaatcggctgcgggcgaattgctcagattttaaaggcctccgcaccatcaggcagatgatttatcagccacacgcgtttgaggctgtgactcatggatatttttatcaaagggcgcgagatagcgagcgtgcccttgcacgcggctcgattaatggtgaaaggcgctggcctgcaaaatttagtttatggtacttcaggccgcagggggactgtccagcccagtggtataaccagccgtttgtggccagatttaagtcacactgcttttatcagccgacggcggagacgtgtgaaaatgtatataacacattttag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z