BBa_K174015
1
BBa_K174015
Cadmium Sensor
2009-10-13T11:00:00Z
2015-05-08T01:10:58Z
CadA promoter which has the binding site for CzrA protein is combined with ArsR binding site to create this AND gate Cadmium sensor.
This Cadmium sensor has binding sites for ArsR and CzrA metal sensors. By placing them together, our device works as an AND gate and senses Cadmium when both proteins bind to Cadmium.
When Cadmium is not present both proteins bind to their relevant sites on the promoter and represses the expression of downstream genes. When Cadmium present in the environment, it binds to these repressors which then fall of from where they are bound to on the promoter. Hence the promoter becomes free to express the downstream genes as an indication of Cadmium.
This combinatorial approach enables us to filter out sensing other metals. ArsR and CzrA can bind to different metals, however when both are used as an ANd gate, they will only recognize Cadmium.
false
false
_277_
0
3942
9
It's complicated
true
We wanted to sense Cadmium accurately and used a combinatorial approach to achieve our goal.
false
The Newcastle 2009 iGEM team
component2040856
1
BBa_K174017
component2040851
1
BBa_K174016
annotation2040851
1
BBa_K174016
range2040851
1
1
26
annotation2040856
1
BBa_K174017
range2040856
1
35
205
BBa_K174016
1
BBa_K174016
Promoterless ArsR binding site
2009-10-13T11:00:00Z
2015-07-24T01:17:49Z
''B. subtilis''
This part purely holds the binding site for ArsR protein which can be released when bound to different metals. Hence this part can be combined with any promoter to sense metals that can bind to ArsR protein. When added in front of a promoter with another metal sensor's protein's binding site, it can form an AND gate to sensitively detect specific metals.
false
false
_277_
4206
3942
9
Not in stock
false
It was developed mainly to use combinatorial approaches for metal sensing.
false
The Newcastle 2009 iGEM team
annotation2040774
1
ArsR binding site
range2040774
1
9
26
BBa_K174017
1
BBa_K174017
CadA promoter with CzrA binding site
2009-10-13T11:00:00Z
2015-07-24T01:17:17Z
It is taken from ''B. subtilis'' ''cadA'' promoter.
In ''B. subtilis'' CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by CzrA repressor protein. When certain metals get inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system.
By taking the promoter of CadA system, we created a generic metal sensor. Hence when it is combined with coding sequences of other genes, it will regulate the expression of these genes upon sensing metals.
false
false
_277_
4206
3942
9
Not in stock
false
It was developed mainly to use combinatorial approaches for metal sensing
false
The Newcastle 2009 iGEM team
annotation2040829
1
sigA -10
range2040829
1
42
47
annotation2040830
1
CzrA binding site
range2040830
1
56
86
annotation2040827
1
cadA promoter
range2040827
1
11
144
annotation2040828
1
sigA -35
range2040828
1
18
23
BBa_K174017_sequence
1
gcgcttgctgtttttcattgacactttcttggaaaacaacatataataggtgtaacttatatatgagtatatgctcatatatataaaataaatacaatactcattgatacgctttgaagagggaaccctaaatcggaaggtaagctaagaggaggaactactatggctagc
BBa_K174015_sequence
1
agtaatcaaaataaattgatttattttactagaggcgcttgctgtttttcattgacactttcttggaaaacaacatataataggtgtaacttatatatgagtatatgctcatatatataaaataaatacaatactcattgatacgctttgaagagggaaccctaaatcggaaggtaagctaagaggaggaactactatggctagc
BBa_K174016_sequence
1
agtaatcaaaataaattgatttattt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z