BBa_K1741000 1 BBa_K1741000 sfGFP under arabinose promoter s1 without AraC (Arashort1) 2015-09-16T11:00:00Z 2015-09-17T04:48:14Z All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section. In commercially available expression vectors arabinose promoter araBAD is usually used together with a gene for AraC transcription activator / repressor to make the system independent of chromosomal copy of AraC gene. We fused the araBAD promoter without AraC reading frame, as other sugar induced promoters to sfGFP. The shorter version of the popular promoter is still functional i.e. induced by arabinose. false false _2163_ 26053 26053 9 false We fused the araBAD promoter without AraC reading frame, as other sugar induced promoters to sfGFP. false Marta Żardecka annotation2467257 1 5'UTR range2467257 1 283 309 annotation2467240 1 -35 signal range2467240 1 245 249 annotation2467258 1 His-tag range2467258 1 310 339 annotation2467268 1 sfGFP range2467268 1 340 1053 annotation2467239 1 I1, I2 binding site range2467239 1 215 247 annotation2467236 1 O2 region range2467236 1 1 16 annotation2467259 1 two consecutive STOP codons range2467259 1 1054 1059 annotation2467256 1 -10 signal range2467256 1 269 273 annotation2467238 1 CAP binding site range2467238 1 203 213 annotation2467237 1 O1 region range2467237 1 158 179 BBa_K1741000_sequence 1 aaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttggatggagtgaaacgatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z