LOCUS       BBa_K1741002             877 bp    DNA     linear       09-Jan-2025
DEFINITION  sfGFP under a short arabinose promoter s2 without O1 and O2
ACCESSION   BBa_K1741002
VERSION     BBa_K1741002.1  
FEATURES             Location/Qualifiers
     misc_feature    21..31
                     /label=CRP binding site
     misc_feature    23..65
                     /label=I1, I2 binding site
     misc_feature    63..67
                     /label=-35 signal
     misc_feature    87..91
                     /label=-10 signal
     misc_feature    101..127
                     /label=5'UTR
     misc_feature    128..157
                     /label=His-tag
     misc_feature    872..877
                     /label=two consecutive STOP codons
     CDS             158..871
                     /label=sfGFP
ORIGIN
        1 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
       61 acctgacgct ttttatcgca actctctact gtttctccat acccgttttt ttggatggag
      121 tgaaacgatg ggcagcagcc atcatcatca tcatcatatg cgtaaaggcg aagagctgtt
      181 cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc ataagttttc
      241 cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga agttcatctg
      301 tactactggt aaactgccgg taccttggcc gactctggta acgacgctga cttatggtgt
      361 tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat
      421 gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac
      481 gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat
      541 tgactttaaa gaagacggca atatcctggg ccataagctg gaatacaatt ttaacagcca
      601 caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg
      661 ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa acactccaat
      721 cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa gcgttctgtc
      781 taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg
      841 catcacgcat ggtatggatg aactgtacaa ataataa
//