BBa_K1741002 1 BBa_K1741002 sfGFP under a short arabinose promoter s2 without O1 and O2 2015-09-16T11:00:00Z 2015-09-17T04:48:49Z All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section. This, shortened version of araBAD promoter does not contain O1 and O2 regions, but still contains the CRP binding site. false false _2163_ 26053 26053 9 false We removed O1 and O2 regions from the promoter. false Marta Żardecka annotation2467262 1 -35 signal range2467262 1 63 67 annotation2467261 1 I1, I2 binding site range2467261 1 23 65 annotation2467264 1 5'UTR range2467264 1 101 127 annotation2467263 1 -10 signal range2467263 1 87 91 annotation2467260 1 CRP binding site range2467260 1 21 31 annotation2467269 1 sfGFP range2467269 1 158 871 annotation2467266 1 two consecutive STOP codons range2467266 1 872 877 annotation2467265 1 His-tag range2467265 1 128 157 BBa_K1741002_sequence 1 ttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttggatggagtgaaacgatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z