LOCUS       BBa_K1741003             913 bp    DNA     linear       23-Dec-2024
DEFINITION  sfGFP under melibiose promoter
ACCESSION   BBa_K1741003
VERSION     BBa_K1741003.1  
FEATURES             Location/Qualifiers
     misc_feature    28..45
                     /label=MelR binding site 1
     misc_feature    48..63
                     /label=CRP binding site
     misc_feature    66..84
                     /label=MelR binding site 1
     misc_feature    102..107
                     /label=-35 signal
     misc_feature    125..129
                     /label=-10 signal
     misc_feature    138..163
                     /label=5'UTR
     RBS             151..156
                     /label=RBS
     misc_feature    164..193
                     /label=His-tag
     CDS             194..907
                     /label=sfGFP
     misc_feature    908..913
                     /label=two consecutive STOP codons
ORIGIN
        1 gccgaaaact ctgcttttca ggtaatttat tcccataaac tcagatttac tgctgcttca
       61 cgcaggatct gagtttatgg gaatgctcaa cctggaagcc ggaggttttc tggagattcg
      121 cctgccatga tgaagttatt caagcaagcc aggagatctg catatgggca gcagccatca
      181 tcatcatcat catatgcgta aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt
      241 ggaactggat ggtgatgtca acggtcataa gttttccgtg cgtggcgagg gtgaaggtga
      301 cgcaactaat ggtaaactga cgctgaagtt catctgtact actggtaaac tgccggtacc
      361 ttggccgact ctggtaacga cgctgactta tggtgttcag tgctttgctc gttatccgga
      421 ccatatgaag cagcatgact tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg
      481 cacgatttcc tttaaggatg acggcacgta caaaacgcgt gcggaagtga aatttgaagg
      541 cgataccctg gtaaaccgca ttgagctgaa aggcattgac tttaaagaag acggcaatat
      601 cctgggccat aagctggaat acaattttaa cagccacaat gtttacatca ccgccgataa
      661 acaaaaaaat ggcattaaag cgaattttaa aattcgccac aacgtggagg atggcagcgt
      721 gcagctggct gatcactacc agcaaaacac tccaatcggt gatggtcctg ttctgctgcc
      781 agacaatcac tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga
      841 tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc acgcatggta tggatgaact
      901 gtacaaataa taa
//