BBa_K1741003 1 BBa_K1741003 sfGFP under melibiose promoter 2015-09-16T11:00:00Z 2015-09-17T05:30:32Z All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section. MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters. false false _2163_ 26053 26053 9 false None. false Marta Żardecka annotation2467292 1 two consecutive STOP codons range2467292 1 908 913 annotation2467279 1 CRP binding site range2467279 1 48 63 annotation2467286 1 RBS range2467286 1 151 156 annotation2467287 1 His-tag range2467287 1 164 193 annotation2467291 1 sfGFP range2467291 1 194 907 annotation2467283 1 5'UTR range2467283 1 138 163 annotation2467282 1 -10 signal range2467282 1 125 129 annotation2467281 1 -35 signal range2467281 1 102 107 annotation2467280 1 MelR binding site 1 range2467280 1 66 84 annotation2467267 1 MelR binding site 1 range2467267 1 28 45 BBa_K1741003_sequence 1 gccgaaaactctgcttttcaggtaatttattcccataaactcagatttactgctgcttcacgcaggatctgagtttatgggaatgctcaacctggaagccggaggttttctggagattcgcctgccatgatgaagttattcaagcaagccaggagatctgcatatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z