LOCUS       BBa_K1741005             906 bp    DNA     linear       27-Sep-2024
DEFINITION  sfGFP under rhamnose promoter with removed EcoRI site
ACCESSION   BBa_K1741005
VERSION     BBa_K1741005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=CRP binding site
     misc_feature    30..51
                     /label=CRP binding site
     misc_feature    52..68
                     /label=RhaS binding site
     misc_feature    85..101
                     /label=RhaS binding site
     misc_feature    99..104
                     /label=-35 signal
     misc_feature    122..127
                     /label=-10 signal
     misc_feature    138..156
                     /label=modified 5
     misc_feature    157..186
                     /label=His-tag
     CDS             187..900
                     /label=sfGFP
     misc_feature    901..906
                     /label=two consecutive STOP codons
ORIGIN
        1 ggtgagcatc acatcaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc
       61 tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattt aggcgctttt
      121 tagactggtc gtaatgaaat tcagcaggat cacattatgg gcagcagcca tcatcatcat
      181 catcatatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg
      241 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact
      301 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg
      361 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg
      421 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt
      481 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc
      541 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc
      601 cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa
      661 aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcagctg
      721 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat
      781 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg
      841 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa
      901 taataa
//