BBa_K1741005 1 BBa_K1741005 sfGFP under rhamnose promoter with removed EcoRI site 2015-09-16T11:00:00Z 2015-09-18T05:57:18Z All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section. rhaBAD promoter has been copied from E. coli genome: ggtgagcatcacatcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcGAATTCaggcgctttttagactggtcgtaatgaaattcagcaggatcacatt then Eco RI site was removed by PCR to obtain Rha1 in a biobrick standard: ggtgagcatcacatcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcGAATTTaggcgctttttagactggtcgtaatgaaattcagcaggatcacatt The only one point mutation makes the promoter slightly stronger and more sensitive to rhamnose. false false _2163_ 26053 26053 9 true We have removed EcoRI site. false Marta Żardecka annotation2467318 1 CRP binding site range2467318 1 1 19 annotation2467325 1 His-tag range2467325 1 157 186 annotation2467322 1 -35 signal range2467322 1 99 104 annotation2467320 1 RhaS binding site range2467320 1 52 68 annotation2467319 1 CRP binding site range2467319 1 30 51 annotation2467321 1 RhaS binding site range2467321 1 85 101 annotation2467323 1 -10 signal range2467323 1 122 127 annotation2467327 1 two consecutive STOP codons range2467327 1 901 906 annotation2467326 1 sfGFP range2467326 1 187 900 annotation2467324 1 modified 5 range2467324 1 138 156 BBa_K1741005_sequence 1 ggtgagcatcacatcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaatttaggcgctttttagactggtcgtaatgaaattcagcaggatcacattatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z