BBa_K1741005
1
BBa_K1741005
sfGFP under rhamnose promoter with removed EcoRI site
2015-09-16T11:00:00Z
2015-09-18T05:57:18Z
All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.
rhaBAD promoter has been copied from E. coli genome: ggtgagcatcacatcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcGAATTCaggcgctttttagactggtcgtaatgaaattcagcaggatcacatt
then Eco RI site was removed by PCR
to obtain Rha1 in a biobrick standard:
ggtgagcatcacatcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcGAATTTaggcgctttttagactggtcgtaatgaaattcagcaggatcacatt
The only one point mutation makes the promoter slightly stronger and more sensitive to rhamnose.
false
false
_2163_
26053
26053
9
true
We have removed EcoRI site.
false
Marta Żardecka
annotation2467318
1
CRP binding site
range2467318
1
1
19
annotation2467325
1
His-tag
range2467325
1
157
186
annotation2467322
1
-35 signal
range2467322
1
99
104
annotation2467320
1
RhaS binding site
range2467320
1
52
68
annotation2467319
1
CRP binding site
range2467319
1
30
51
annotation2467321
1
RhaS binding site
range2467321
1
85
101
annotation2467323
1
-10 signal
range2467323
1
122
127
annotation2467327
1
two consecutive STOP codons
range2467327
1
901
906
annotation2467326
1
sfGFP
range2467326
1
187
900
annotation2467324
1
modified 5
range2467324
1
138
156
BBa_K1741005_sequence
1
ggtgagcatcacatcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaatttaggcgctttttagactggtcgtaatgaaattcagcaggatcacattatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z