LOCUS       BBa_K1741006             855 bp    DNA     linear       26-Nov-2024
DEFINITION  sfGFP under short rhamnose promoter (very weak)
ACCESSION   BBa_K1741006
VERSION     BBa_K1741006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=RhaS binding site
     misc_feature    34..50
                     /label=RhaS binding site
     misc_feature    48..53
                     /label=-35 signal
     misc_feature    71..76
                     /label=-10 signal
     misc_feature    87..105
                     /label=modified 5'UTR
     misc_feature    106..135
                     /label=His-tag
     CDS             136..849
                     /label=sfGFP
     misc_feature    850..855
                     /label=two consecutive STOP codons
ORIGIN
        1 atctttccct ggttgccaat ggcccatttt cctgtcagta acgagaaggt cgcgaattta
       61 ggcgcttttt agactggtcg taatgaaatt cagcaggatc acattatggg cagcagccat
      121 catcatcatc atcatatgcg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg
      181 gtggaactgg atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt
      241 gacgcaacta atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta
      301 ccttggccga ctctggtaac gacgctgact tatggtgttc agtgctttgc tcgttatccg
      361 gaccatatga agcagcatga cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa
      421 cgcacgattt cctttaagga tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa
      481 ggcgataccc tggtaaaccg cattgagctg aaaggcattg actttaaaga agacggcaat
      541 atcctgggcc ataagctgga atacaatttt aacagccaca atgtttacat caccgccgat
      601 aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc acaacgtgga ggatggcagc
      661 gtgcagctgg ctgatcacta ccagcaaaac actccaatcg gtgatggtcc tgttctgctg
      721 ccagacaatc actatctgag cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc
      781 gatcatatgg ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa
      841 ctgtacaaat aataa
//