BBa_K1741006 1 BBa_K1741006 sfGFP under short rhamnose promoter (very weak) 2015-09-16T11:00:00Z 2015-09-26T08:00:23Z All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section. From rhaBAD1 promoter we removed both CRP binding sites. It seems likely thatt RhaS binding site has been also affected, so however the resulting promoter is still induced by rhamnose, the induction is very weak. false false _2163_ 26053 26053 9 false We removed both CPR sites. false Marta Żardecka annotation2467328 1 RhaS binding site range2467328 1 1 17 annotation2467334 1 sfGFP range2467334 1 136 849 annotation2467332 1 modified 5'UTR range2467332 1 87 105 annotation2467333 1 His-tag range2467333 1 106 135 annotation2467330 1 -35 signal range2467330 1 48 53 annotation2467331 1 -10 signal range2467331 1 71 76 annotation2467335 1 two consecutive STOP codons range2467335 1 850 855 annotation2467329 1 RhaS binding site range2467329 1 34 50 BBa_K1741006_sequence 1 atctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgaatttaggcgctttttagactggtcgtaatgaaattcagcaggatcacattatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z