LOCUS       BBa_K1741008            1126 bp    DNA     linear       05-Feb-2026
DEFINITION  sfGFP under promoter xylA1 with improved 5'UTR
ACCESSION   BBa_K1741008
VERSION     BBa_K1741008.1  
FEATURES             Location/Qualifiers
     misc_feature    235..253
                     /label=CRP binding site
     misc_feature    254..268
                     /label=XylR binding site
     misc_feature    274..289
                     /label=XylR binding site
     misc_feature    290..294
                     /label=-35 signal
     misc_feature    313..318
                     /label=-10 signal
     misc_feature    324..376
                     /label=modified 5'UTR
     misc_feature    338..376
                     /label=part of 5'UTR taken from proD promoter
     misc_feature    377..406
                     /label=His-tag
     CDS             407..1120
                     /label=sfGFP
     misc_feature    1121..1126
                     /label=two consecutive STOP codons
ORIGIN
        1 ggtgtagggc cttctgtagt tagaggacag ttttaataag taacaatcac cgcgataaac
       61 gtaaccaatt tttagcaact aaacagggga aaacaattac agatttttat ctttcgatta
      121 cgatttttgg tttatttctt gatttatgac cgagatctta cttttgttgc gcaattgtac
      181 ttattgcatt tttctcttcg aggaattacc cagtttcatc attccatttt attttgcgag
      241 cgagcgcaca cttgtgaatt atctcaatag cagtgtgaaa taacataatt gagcaactga
      301 aagggagtgc ccaatattac gacatcatcc atcaccctct acaaataatt ttgtttaact
      361 ttgaggaggt tatcatatgg gcagcagcca tcatcatcat catcatatgc gtaaaggcga
      421 agagctgttc actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca
      481 taagttttcc gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa
      541 gttcatctgt actactggta aactgccggt accttggccg actctggtaa cgacgctgac
      601 ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa
      661 gtccgccatg ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac
      721 gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct
      781 gaaaggcatt gactttaaag aagacggcaa tatcctgggc cataagctgg aatacaattt
      841 taacagccac aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt
      901 taaaattcgc cacaacgtgg aggatggcag cgtgcagctg gctgatcact accagcaaaa
      961 cactccaatc ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag
     1021 cgttctgtct aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac
     1081 cgcagcgggc atcacgcatg gtatggatga actgtacaaa taataa
//