LOCUS       BBa_K1741009             892 bp    DNA     linear       23-Dec-2024
DEFINITION  sfGFP under shortened promoter xylA1 with improved 5'UTR
ACCESSION   BBa_K1741009
VERSION     BBa_K1741009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=CRP binding site
     misc_feature    20..34
                     /label=XylR binding site
     misc_feature    40..55
                     /label=XylR binding site
     misc_feature    56..60
                     /label=-35 signal
     misc_feature    79..84
                     /label=-10 signal
     misc_feature    90..142
                     /label=modified 5'UTR
     misc_feature    104..142
                     /label=part of 5'UTR taken from proD promoter
     misc_feature    143..172
                     /label=His-tag
     CDS             173..886
                     /label=sfGFP
     misc_feature    887..892
                     /label=two consecutive STOP codons
ORIGIN
        1 tgcgagcgag cgcacacttg tgaattatct caatagcagt gtgaaataac ataattgagc
       61 aactgaaagg gagtgcccaa tattacgaca tcatccatca ccctctacaa ataattttgt
      121 ttaactttga ggaggttatc atatgggcag cagccatcat catcatcatc atatgcgtaa
      181 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
      241 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
      301 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac
      361 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
      421 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
      481 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat
      541 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata
      601 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc
      661 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
      721 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac
      781 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
      841 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaataat aa
//