BBa_K1741009 1 BBa_K1741009 sfGFP under shortened promoter xylA1 with improved 5'UTR 2015-09-16T11:00:00Z 2015-09-17T07:24:06Z All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section. Short version of xylA promoter with known functional elements is stronger than two other versions (BBa_K1741007, BBa_K1741008). false false _2163_ 26053 26053 9 false None. false Marta Żardecka annotation2467408 1 sfGFP range2467408 1 173 886 annotation2467401 1 modified 5'UTR range2467401 1 90 142 annotation2467410 1 two consecutive STOP codons range2467410 1 887 892 annotation2467406 1 His-tag range2467406 1 143 172 annotation2467400 1 -10 signal range2467400 1 79 84 annotation2467396 1 XylR binding site range2467396 1 20 34 annotation2467398 1 -35 signal range2467398 1 56 60 annotation2467395 1 CRP binding site range2467395 1 1 19 annotation2467397 1 XylR binding site range2467397 1 40 55 annotation2467405 1 part of 5'UTR taken from proD promoter range2467405 1 104 142 BBa_K1741009_sequence 1 tgcgagcgagcgcacacttgtgaattatctcaatagcagtgtgaaataacataattgagcaactgaaagggagtgcccaatattacgacatcatccatcaccctctacaaataattttgtttaactttgaggaggttatcatatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z