BBa_K1741010
1
BBa_K1741010
sfGFP under xylose promoter xylF (very weak)
2015-09-16T11:00:00Z
2015-09-17T06:33:37Z
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.
We also tested the xylF promoter from the same double sided regulatory element (cf. BBa_K1741007), and xylF appears to be a very weak promoter slightly induced by xylose.
false
false
_2163_
26053
26053
9
false
None.
false
Marta Żardecka
annotation2467420
1
His-Tag
range2467420
1
181
210
annotation2467412
1
IF1
range2467412
1
43
58
annotation2467416
1
-10 signal
range2467416
1
107
112
annotation2467421
1
sfGFP
range2467421
1
211
924
annotation2467414
1
IF2
range2467414
1
63
78
annotation2467418
1
5'UTR
range2467418
1
119
180
annotation2467426
1
two consecutive STOP codons
range2467426
1
925
930
annotation2467415
1
-35 signal
range2467415
1
86
90
BBa_K1741010_sequence
1
gtacaattgcgcaacaaaagtaagatctcggtcataaatcaagaaataaaccaaaaatcgtaatcgaaagataaaaatctgtaattgttttcccctgtttagttgctaaaaattggttacgtttatcgcggtgattgttacttattaaaactgtcctctaactacagaaggccctacaccatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z