LOCUS       BBa_K1741011             886 bp    DNA     linear       22-Dec-2024
DEFINITION  sfGFP under T7 promoter
ACCESSION   BBa_K1741011
VERSION     BBa_K1741011.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=T7 promoter
     misc_feature    839..886
                     /label=T7 terminator
     misc_feature    21..44
                     /label=operator lac
     misc_feature    89..118
                     /label=His-tag
     CDS             119..832
                     /label=sfGFP
     misc_feature    833..838
                     /label=two consecutive STOP codons
     misc_feature    20..88
                     /label=5'UTR
ORIGIN
        1 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
       61 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcatat
      121 gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac tggatggtga
      181 tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa
      241 actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc cgactctggt
      301 aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccata tgaagcagca
      361 tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga tttcctttaa
      421 ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa
      481 ccgcattgag ctgaaaggca ttgactttaa agaagacggc aatatcctgg gccataagct
      541 ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa aaaatggcat
      601 taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc tggctgatca
      661 ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca atcactatct
      721 gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata tggttctgct
      781 ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca aataataact
      841 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttg
//