BBa_K1741012
1
BBa_K1741012
sfGFP under proC promoter
2015-09-16T11:00:00Z
2015-09-17T06:48:58Z
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.
proC moderately strong constitutive promoter (cf. Davis 2010) seems to reflect growth rate of E. coli bacterial strains on different media, and potentially can be a measure of protein biosynthesis rate, to be determined in future... Expression is higher than of inducible promoters
false
false
_2163_
26053
26053
9
false
None.
false
Marta Żardecka
annotation2467521
1
sfGFP
range2467521
1
189
902
annotation2467520
1
His-tag
range2467520
1
159
188
annotation2467502
1
-35 signal
range2467502
1
63
68
annotation2467501
1
proC promoter
range2467501
1
1
101
annotation2467503
1
-10 signal
range2467503
1
86
91
annotation2467522
1
two consecutive STOP codons
range2467522
1
903
908
annotation2467504
1
5'UTR
range2467504
1
102
158
BBa_K1741012_sequence
1
cacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtatgatatattcagggagaccacaacggtttccctctacaaataattttgtttaactttgaggaggttatcatatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z