LOCUS       BBa_K1741013             939 bp    DNA     linear       26-Dec-2024
DEFINITION  sfGFP under proC promoter with a hairpin in 5'UTR
ACCESSION   BBa_K1741013
VERSION     BBa_K1741013.1  
FEATURES             Location/Qualifiers
     promoter        1..101
                     /label=proC promoter
     misc_feature    63..68
                     /label=-35 signal
     misc_feature    86..91
                     /label=-10 signal
     misc_feature    102..189
                     /label=5'UTR with hairpin
     misc_feature    145..175
                     /label=hairpin
     misc_feature    190..219
                     /label=His-tag
     CDS             220..933
                     /label=sfGFP
     misc_feature    934..939
                     /label=two consectuive STOP codons
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa ctttaatata tagacactga aaagtgtctt tttttgagga
      181 ggttatcata tgggcagcag ccatcatcat catcatcata tgcgtaaagg cgaagagctg
      241 ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt
      301 tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc
      361 tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct gacttatggt
      421 gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc
      481 atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa
      541 acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc
      601 attgacttta aagaagacgg caatatcctg ggccataagc tggaatacaa ttttaacagc
      661 cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt
      721 cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca
      781 atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg
      841 tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg
      901 ggcatcacgc atggtatgga tgaactgtac aaataataa
//