BBa_K1741013 1 BBa_K1741013 sfGFP under proC promoter with a hairpin in 5'UTR 2015-09-16T11:00:00Z 2015-09-17T07:03:26Z All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section. A weak hairpin introduced to 5???UTR slightly lowers transcription/translation. Will be extended to a thermometer, actually we see no temp. dependence. false false _2163_ 26053 26053 9 false A weak hairpin introduced to 5'UTR. false Marta Żardecka annotation2467535 1 sfGFP range2467535 1 220 933 annotation2467532 1 5'UTR with hairpin range2467532 1 102 189 annotation2467527 1 -35 signal range2467527 1 63 68 annotation2467536 1 two consectuive STOP codons range2467536 1 934 939 annotation2467526 1 proC promoter range2467526 1 1 101 annotation2467534 1 His-tag range2467534 1 190 219 annotation2467533 1 hairpin range2467533 1 145 175 annotation2467531 1 -10 signal range2467531 1 86 91 BBa_K1741013_sequence 1 cacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtatgatatattcagggagaccacaacggtttccctctacaaataattttgtttaactttaatatatagacactgaaaagtgtctttttttgaggaggttatcatatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z