BBa_K1742003 1 BBa_K1742003 Dronpa 145K 2015-09-03T11:00:00Z 2016-01-25T11:27:33Z Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x From Pectinidae sp. It is a CDS of green fluorescent protein Dronpa. false false _2164_ 4206 26598 9 false It is codon optimized for plants. false Iv??n Casas Rodrigo BBa_K1742002 1 BBa_K1742002 Dronpa 145N 2015-09-03T11:00:00Z 2016-01-25T11:27:00Z Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x A CDS of Dronpa protein with a mutation in the betha-strand 7 in Lys145 for Asn. false false _2164_ 4206 26598 9 false It is codon optimized for plants false Iv??n Casas Rodrigo BBa_K1742010 1 BBa_K1742010 35S:LexABD-DronpaK:T35S - 35S:DronpaN-VP16:T35S 2015-09-06T11:00:00Z 2015-12-16T01:54:14Z It has been synthesis. Composite composed of the two transcriptional units needed to obtain a toggle switch control by violet and cian light. false false _2164_ 4206 26679 9 false Hola false Pilar Baldominos component2445529 1 BBa_K1742002 component2445528 1 BBa_K1537015 component2445524 1 BBa_K1742003 component2445523 1 BBa_K1537015 annotation2445523 1 BBa_K1537015 range2445523 1 1 860 annotation2445528 1 BBa_K1537015 range2445528 1 1618 2477 annotation2445529 1 BBa_K1742002 range2445529 1 2484 3176 annotation2445524 1 BBa_K1742003 range2445524 1 869 1609 BBa_K1537015 1 BBa_K1537015 35S promoter+translation initiation optimized sequence for dicot+Mass translation enhancer 2014-08-17T11:00:00Z 2015-05-08T01:10:52Z The 35S promoter has a large area of application in genetically modified plants.The translation initiation optimized sequence for dicot is from the paper "translation initiation optimized sequence for dicot"(Nucleic Acids Research Volume 36, Issue 3Pp. 861-871 So Nakagawa1, Yoshihito Niimura2, Takashi Gojobori3,4, Hiroshi Tanaka1,2,* and Kin-ichiro Miura5) The 35S promoter is a strong promoter derived from cauliflower mosaic virus. This constitutive promoter is widely used in transgenic plants to improve the level of the expression of foreign genes effectively."AAAAAAATG" was a kind of preferred nucleotide sequence used in dicot plants（ATG is the initiation codon）to enhance translation initiation.And insertion of ???GCT TCC TCC???(mass translation enhancer) after the initiator codon ATG can augment downstream gene-expression in plants. false false _1919_ 0 22415 9 It's complicated false We have to remove some restriction endonuclease cutting sites by changing a few bases. false Jie Li annotation2393797 1 35s promoter range2393797 1 1 842 annotation2393798 1 translation initiation optimized sequence for dicot range2393798 1 843 851 annotation2393799 1 mass translation enhancer range2393799 1 852 860 BBa_K1537015_sequence 1 agatttgccttttcaatttcagaaagaatgctaacccacagatggttagagaggcttacgcagcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggggactaaaaaaatggcttcctcc BBa_K1742003_sequence 1 ggaggaggaggatctggaggaggaggatctggaggaggaggatctatgtctgtgatcaagcctgatatgaagatcaagctcagaatggaaggtgctgttaacggacatcctttcgctattgagggtgttggactcggaaagccttttgagggaaagcagtctatggatctcaaggtgaaagaaggtggacctcttcctttcgcttacgatattctcactactgtgttctgctacggaaacagagtgttcgctaagtaccctgagaacatcgtggattacttcaagcagtctttccctgaaggatactcttgggagagatcaatgaactacgaggatggtggaatctgtaacgctactaacgatattactctcgatggtgattgctacatctacgagatcagattcgatggtgttaacttccctgctaacggacctgttatgcaaaagcgtactgtgaagtgggaaccttctactgagaagctctatgtgagagatggtgttctcaagggtgatgtgaacatggctctctcacttgaaggtggaggacactaccgttgcgatttcaagactacttacaaggctaagaaagtggttcagctccctgattaccatttcgtggatcaccacatcgagatcaagtctcacgataaggattactctaacgtgaacctccatgaacatgctgaagctcactctgagcttcctagacaggctaagcccaagaagaagaggaaggtgtga BBa_K1742010_sequence 1 agatttgccttttcaatttcagaaagaatgctaacccacagatggttagagaggcttacgcagcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggggactaaaaaaatggcttcctcctactagagggaggaggaggatctggaggaggaggatctggaggaggaggatctatgtctgtgatcaagcctgatatgaagatcaagctcagaatggaaggtgctgttaacggacatcctttcgctattgagggtgttggactcggaaagccttttgagggaaagcagtctatggatctcaaggtgaaagaaggtggacctcttcctttcgcttacgatattctcactactgtgttctgctacggaaacagagtgttcgctaagtaccctgagaacatcgtggattacttcaagcagtctttccctgaaggatactcttgggagagatcaatgaactacgaggatggtggaatctgtaacgctactaacgatattactctcgatggtgattgctacatctacgagatcagattcgatggtgttaacttccctgctaacggacctgttatgcaaaagcgtactgtgaagtgggaaccttctactgagaagctctatgtgagagatggtgttctcaagggtgatgtgaacatggctctctcacttgaaggtggaggacactaccgttgcgatttcaagactacttacaaggctaagaaagtggttcagctccctgattaccatttcgtggatcaccacatcgagatcaagtctcacgataaggattactctaacgtgaacctccatgaacatgctgaagctcactctgagcttcctagacaggctaagcccaagaagaagaggaaggtgtgatactagagagatttgccttttcaatttcagaaagaatgctaacccacagatggttagagaggcttacgcagcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggggactaaaaaaatggcttcctcctactagatgagtgtgattaagcccgatatgaagattaaactacggatggaaggtgctgtgaacggtcatccttttgctattgagggagtgggtttgggcaagccattcgagggcaaacagtctatggatttaaaagtgaaggagggagggcctttgccttttgcatatgatatactcaccacggttttttgttatggtaatcgtgtctttgcaaaatatcctgagaacattgtggattattttaaacaaagtttccccgagggatattcatgggagaggagtatgaattatgaagatgggggaatttgtaatgctaccaatgacatcactttggacggggactgttacatttatgagatacgtttcgatggagttaatttcccagccaatgggcccgtcatgcagaaaaggacggtgaagtgggaaccatccacagagaacctgtacgtcagagatggagttcttaagggagatgttaatatggccttgtctttggaaggtgggggacattatcgttgtgacttcaagacaacttataaagcaaaaaaggtcgttcagttacctgattatcactttgtggatcatcacatcgagataaagtcacacgataaggactactctaatgttaatcttcatgagcatgcagaagcccattccgagcttccaaggcaggctaaacctaaaaagaagagaaaagtt BBa_K1742002_sequence 1 atgagtgtgattaagcccgatatgaagattaaactacggatggaaggtgctgtgaacggtcatccttttgctattgagggagtgggtttgggcaagccattcgagggcaaacagtctatggatttaaaagtgaaggagggagggcctttgccttttgcatatgatatactcaccacggttttttgttatggtaatcgtgtctttgcaaaatatcctgagaacattgtggattattttaaacaaagtttccccgagggatattcatgggagaggagtatgaattatgaagatgggggaatttgtaatgctaccaatgacatcactttggacggggactgttacatttatgagatacgtttcgatggagttaatttcccagccaatgggcccgtcatgcagaaaaggacggtgaagtgggaaccatccacagagaacctgtacgtcagagatggagttcttaagggagatgttaatatggccttgtctttggaaggtgggggacattatcgttgtgacttcaagacaacttataaagcaaaaaaggtcgttcagttacctgattatcactttgtggatcatcacatcgagataaagtcacacgataaggactactctaatgttaatcttcatgagcatgcagaagcccattccgagcttccaaggcaggctaaacctaaaaagaagagaaaagtt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z