BBa_K1742003 1 BBa_K1742003 Dronpa 145K 2015-09-03T11:00:00Z 2016-01-25T11:27:33Z Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x From Pectinidae sp. It is a CDS of green fluorescent protein Dronpa. false false _2164_ 4206 26598 9 false It is codon optimized for plants. false Iv??n Casas Rodrigo BBa_K1467101 1 BBa_K1467101 GoldenGate compatible 35s Promoter 2014-09-23T11:00:00Z 2015-05-29T01:36:07Z Cauliflower Mosaic Virus (CaMV) 35S is a plant specific promoter obtained from Cauliflower Mosaic Virus. The part is intended for use as a constitutive promoter for gene expression experiments. The part is compatible with GoldenGate MoClo Assembly Standard (free from BsaI and BpiI recognition sequences). false false _1846_ 4206 21550 9 In stock false Domestication for GoldenGate (Removal of internal BsaI and BpiI recognition sequences). false Cara Deal BBa_K105007 1 BBa_K105007 Gal4 - DNA binding domain 2008-10-11T11:00:00Z 2015-05-08T01:08:52Z lab plasmid Gal4 is a yeast transcription factor which activates the transcription.<br\n> This BioBrick is the DNA binding domain of Gal4, which binds specificly to the regulatory sequence [[Part:BBa_K105024| BBa_K105024]]. false false _253_ 0 3313 9 In stock true To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006). true Katja Karstens BBa_K1742002 1 BBa_K1742002 Dronpa 145N 2015-09-03T11:00:00Z 2016-01-25T11:27:00Z Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x A CDS of Dronpa protein with a mutation in the betha-strand 7 in Lys145 for Asn. false false _2164_ 4206 26598 9 false It is codon optimized for plants false Iv??n Casas Rodrigo BBa_K105001 1 BBa_K105001 VP16 - eucaryotic activation domain 2008-10-11T11:00:00Z 2015-05-08T01:08:52Z We have constructed our BioBrick from the plasmid xxx, which was available in our hosting lab. Originally VP16 is a protein from the herpes simplex virus. It activates the transcription of the late genes of this virus by the RNA-polymerase II of the eucaryotic host. In artificial systems VP16 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective expression of genes with a promoter containing this bait sequence can be achieved. false false _253_ 0 3313 9 It's complicated true To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. <br\n> For more information about this issus, see Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." DSpace http://hdl.handle.net/1721.1/32535 (2006). true Manuel Gersbacher BBa_K1742012 1 BBa_K1742012 35S:Gal4BD-DronpaK:T35S - 35S:DronpaN-VP16:T35S 2015-09-06T11:00:00Z 2015-12-16T01:55:00Z Synthesis Translational unit composed by the promoter 35S, the T35s terminator, the CDS Dronpa(145K), Dronpa(145N), the binding domain Gal4 and VP16. The CDS works as a toggle switch activated with green light and deactivated with blue light. Dronpa(145K) changes its conformation with light, being able to dimerize with Dronpa(145N), acting as a transcription factor. false false _2164_ 4206 26626 9 false Do a PCR to obtain the Dronpa(145N) false M??nica Victoria Guti??rrez Salazar component2445630 1 BBa_K1467101 component2445632 1 BBa_K1742002 component2445634 1 BBa_K105001 component2445633 1 BBa_K1742003 component2445631 1 BBa_K105007 annotation2445630 1 BBa_K1467101 range2445630 1 1 1432 annotation2445634 1 BBa_K105001 range2445634 1 3335 3610 annotation2445633 1 BBa_K1742003 range2445633 1 2586 3326 annotation2445631 1 BBa_K105007 range2445631 1 1441 1878 annotation2445632 1 BBa_K1742002 range2445632 1 1885 2577 BBa_K1742012_sequence 1 ggaggtattccaatcccacaaaaatctgagcttaacagcacagttgctcctctcagagcagaatcgggtattcaacaccctcatatcaactactacgttgtgtataacggtccacatgccggtatatacgatgactggggttgtacaaaggcggcaacaaacggcgttcccggagttgcacacaagaaatttgccactattacagaggcaagagcagcagctgacgcgtacacaacaagtcagcaaacagacaggttgaacttcatccccaaaggagaagctcaactcaagcccaagagctttgctaaggccctaacaagcccaccaaagcaaaaagcccactggctcacgctaggaaccaaaaggcccagcagtgatccagccccaaaagagatctcctttgccccggagattacaatggacgatttcctctatctttacgatctaggaaggaagttcgaaggtgaaggtgacgacactatgttcaccactgataatgagaaggttagcctcttcaatttcagaaagaatgctgacccacagatggttagagaggcctacgcagcaagtctcatcaagacgatctacccgagtaacaatctccaggagatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaattgcatcaagaacacagagaaagacatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcataaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcctactgaatctaaggccatgcatggagtctaagattcaaatcgaggatctaacagaactcgccgtcaagactggcgaacagttcatacagagtcttttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacactctggtctactccaaaaatgtcaaagatacagtctcagaagatcaaagggctattgagacttttcaacaaaggataatttcgggaaacctcctcggattccattgcccagctatctgtcacttcatcgaaaggacagtagaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggctatcattcaagatctctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagaggttccaaccacgtctacaaagcaagtggattgatgtgacatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagaggacacgctcgagtataagagctcatttttacaacaattaccaacaacaacaaacaacaaacaacattacaattacatttacaattatcgatacaatgtactagagaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgtactagatgagtgtgattaagcccgatatgaagattaaactacggatggaaggtgctgtgaacggtcatccttttgctattgagggagtgggtttgggcaagccattcgagggcaaacagtctatggatttaaaagtgaaggagggagggcctttgccttttgcatatgatatactcaccacggttttttgttatggtaatcgtgtctttgcaaaatatcctgagaacattgtggattattttaaacaaagtttccccgagggatattcatgggagaggagtatgaattatgaagatgggggaatttgtaatgctaccaatgacatcactttggacggggactgttacatttatgagatacgtttcgatggagttaatttcccagccaatgggcccgtcatgcagaaaaggacggtgaagtgggaaccatccacagagaacctgtacgtcagagatggagttcttaagggagatgttaatatggccttgtctttggaaggtgggggacattatcgttgtgacttcaagacaacttataaagcaaaaaaggtcgttcagttacctgattatcactttgtggatcatcacatcgagataaagtcacacgataaggactactctaatgttaatcttcatgagcatgcagaagcccattccgagcttccaaggcaggctaaacctaaaaagaagagaaaagtttactagagggaggaggaggatctggaggaggaggatctggaggaggaggatctatgtctgtgatcaagcctgatatgaagatcaagctcagaatggaaggtgctgttaacggacatcctttcgctattgagggtgttggactcggaaagccttttgagggaaagcagtctatggatctcaaggtgaaagaaggtggacctcttcctttcgcttacgatattctcactactgtgttctgctacggaaacagagtgttcgctaagtaccctgagaacatcgtggattacttcaagcagtctttccctgaaggatactcttgggagagatcaatgaactacgaggatggtggaatctgtaacgctactaacgatattactctcgatggtgattgctacatctacgagatcagattcgatggtgttaacttccctgctaacggacctgttatgcaaaagcgtactgtgaagtgggaaccttctactgagaagctctatgtgagagatggtgttctcaagggtgatgtgaacatggctctctcacttgaaggtggaggacactaccgttgcgatttcaagactacttacaaggctaagaaagtggttcagctccctgattaccatttcgtggatcaccacatcgagatcaagtctcacgataaggattactctaacgtgaacctccatgaacatgctgaagctcactctgagcttcctagacaggctaagcccaagaagaagaggaaggtgtgatactagaggctagcgccgccaccatgggccctaaaaagaagcgtaaagtcgcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtggg BBa_K105007_sequence 1 aagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcg BBa_K105001_sequence 1 gctagcgccgccaccatgggccctaaaaagaagcgtaaagtcgcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtggg BBa_K1467101_sequence 1 ggaggtattccaatcccacaaaaatctgagcttaacagcacagttgctcctctcagagcagaatcgggtattcaacaccctcatatcaactactacgttgtgtataacggtccacatgccggtatatacgatgactggggttgtacaaaggcggcaacaaacggcgttcccggagttgcacacaagaaatttgccactattacagaggcaagagcagcagctgacgcgtacacaacaagtcagcaaacagacaggttgaacttcatccccaaaggagaagctcaactcaagcccaagagctttgctaaggccctaacaagcccaccaaagcaaaaagcccactggctcacgctaggaaccaaaaggcccagcagtgatccagccccaaaagagatctcctttgccccggagattacaatggacgatttcctctatctttacgatctaggaaggaagttcgaaggtgaaggtgacgacactatgttcaccactgataatgagaaggttagcctcttcaatttcagaaagaatgctgacccacagatggttagagaggcctacgcagcaagtctcatcaagacgatctacccgagtaacaatctccaggagatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaattgcatcaagaacacagagaaagacatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcataaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcctactgaatctaaggccatgcatggagtctaagattcaaatcgaggatctaacagaactcgccgtcaagactggcgaacagttcatacagagtcttttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacactctggtctactccaaaaatgtcaaagatacagtctcagaagatcaaagggctattgagacttttcaacaaaggataatttcgggaaacctcctcggattccattgcccagctatctgtcacttcatcgaaaggacagtagaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggctatcattcaagatctctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagaggttccaaccacgtctacaaagcaagtggattgatgtgacatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagaggacacgctcgagtataagagctcatttttacaacaattaccaacaacaacaaacaacaaacaacattacaattacatttacaattatcgatacaatg BBa_K1742003_sequence 1 ggaggaggaggatctggaggaggaggatctggaggaggaggatctatgtctgtgatcaagcctgatatgaagatcaagctcagaatggaaggtgctgttaacggacatcctttcgctattgagggtgttggactcggaaagccttttgagggaaagcagtctatggatctcaaggtgaaagaaggtggacctcttcctttcgcttacgatattctcactactgtgttctgctacggaaacagagtgttcgctaagtaccctgagaacatcgtggattacttcaagcagtctttccctgaaggatactcttgggagagatcaatgaactacgaggatggtggaatctgtaacgctactaacgatattactctcgatggtgattgctacatctacgagatcagattcgatggtgttaacttccctgctaacggacctgttatgcaaaagcgtactgtgaagtgggaaccttctactgagaagctctatgtgagagatggtgttctcaagggtgatgtgaacatggctctctcacttgaaggtggaggacactaccgttgcgatttcaagactacttacaaggctaagaaagtggttcagctccctgattaccatttcgtggatcaccacatcgagatcaagtctcacgataaggattactctaacgtgaacctccatgaacatgctgaagctcactctgagcttcctagacaggctaagcccaagaagaagaggaaggtgtga BBa_K1742002_sequence 1 atgagtgtgattaagcccgatatgaagattaaactacggatggaaggtgctgtgaacggtcatccttttgctattgagggagtgggtttgggcaagccattcgagggcaaacagtctatggatttaaaagtgaaggagggagggcctttgccttttgcatatgatatactcaccacggttttttgttatggtaatcgtgtctttgcaaaatatcctgagaacattgtggattattttaaacaaagtttccccgagggatattcatgggagaggagtatgaattatgaagatgggggaatttgtaatgctaccaatgacatcactttggacggggactgttacatttatgagatacgtttcgatggagttaatttcccagccaatgggcccgtcatgcagaaaaggacggtgaagtgggaaccatccacagagaacctgtacgtcagagatggagttcttaagggagatgttaatatggccttgtctttggaaggtgggggacattatcgttgtgacttcaagacaacttataaagcaaaaaaggtcgttcagttacctgattatcactttgtggatcatcacatcgagataaagtcacacgataaggactactctaatgttaatcttcatgagcatgcagaagcccattccgagcttccaaggcaggctaaacctaaaaagaagagaaaagtt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z