LOCUS       BBa_K1744000            3047 bp    DNA     linear       22-Nov-2024
DEFINITION  araC-PBAD-vcrx028-ampR
ACCESSION   BBa_K1744000
VERSION     BBa_K1744000.1  
FEATURES             Location/Qualifiers
     primer_bind     1..22
                     /label=forward priming site
     CDS             23..901
                     /label=araC
     misc_feature    930..947
                     /label=operator O2
     promoter        1052..1080
                     /label=araC promoter
     misc_feature    1088..1109
                     /label=operator O1
     misc_feature    1131..1144
                     /label=CAP site
     misc_feature    1140..1178
                     /label=operator I2 and I1
     promoter        1177..1204
                     /label=PBAD promoter
     misc_feature    1213..1213
                     /label=TSS
     misc_feature    1205..1205
                     /label=T > G
     RBS             1234..1245
                     /label=RBS
     CDS             1246..1623
                     /label=vcrx028
     misc_feature    1596..1596
                     /label=A > T, was the native stop codon of vcrx028
     stem_loop       1649..2074
                     /label=rrnB terminator T1 T2
     CDS             2365..3027
                     /label=bla
     promoter        2167..2364
                     /label=bla promoter and RBS
     primer_bind     3028..3047
                     /label=reverse priming site
ORIGIN
        1 caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt
       61 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
      121 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
      181 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
      241 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
      301 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
      361 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
      421 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
      481 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
      541 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
      601 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
      661 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
      721 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
      781 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
      841 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
      901 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt
      961 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta
     1021 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
     1081 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
     1141 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
     1201 ctgtgtctcc atacccgttt ttttgggcta gcgtaggagg caaaaatgtg ggtcatcgag
     1261 acaaccgaca cctttgatga gtggttcgat gctctggatg ataccgatag agcaaacgtg
     1321 ctggcttcga tgatggtgct gcgagataga ggccccatgc tgtcgaggcc atacgcggat
     1381 actgttaacg gttcatccta cagcaacatg aaagagcttc gggtccaaag caaaggagat
     1441 cctatcagag cgttctttgc gttcgatcca aagcgtaagg ggattcttct ctgcgccggt
     1501 aacaagaccg gggacgagaa aaggttttat gaagtaatga tcccaattgc agaccgtgag
     1561 tttgcggcgc acttggataa attgaagaag gagtgtcgag ctcggtaccc ggggatcctc
     1621 tagagtcgac ctgcaggcat gcaagcttgg ctgttttggc ggatgagaga agattttcag
     1681 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg
     1741 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
     1801 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac
     1861 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1921 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
     1981 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc
     2041 tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca
     2101 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2161 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2221 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2281 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2341 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2401 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2461 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2521 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2581 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2641 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2701 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2761 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2821 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2881 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2941 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3001 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactc
//