LOCUS BBa_K1744000 3047 bp DNA linear 23-Dec-2024 DEFINITION araC-PBAD-vcrx028-ampR ACCESSION BBa_K1744000 VERSION BBa_K1744000.1 FEATURES Location/Qualifiers primer_bind 1..22 /label=forward priming site CDS 23..901 /label=araC misc_feature 930..947 /label=operator O2 promoter 1052..1080 /label=araC promoter misc_feature 1088..1109 /label=operator O1 misc_feature 1131..1144 /label=CAP site misc_feature 1140..1178 /label=operator I2 and I1 promoter 1177..1204 /label=PBAD promoter misc_feature 1213..1213 /label=TSS misc_feature 1205..1205 /label=T > G RBS 1234..1245 /label=RBS CDS 1246..1623 /label=vcrx028 misc_feature 1596..1596 /label=A > T, was the native stop codon of vcrx028 stem_loop 1649..2074 /label=rrnB terminator T1 T2 CDS 2365..3027 /label=bla promoter 2167..2364 /label=bla promoter and RBS primer_bind 3028..3047 /label=reverse priming site ORIGIN 1 caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt 61 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga 121 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg 181 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa 241 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg 301 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct 361 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca 421 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc 481 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc 541 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc 601 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt 661 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc 721 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc 781 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg 841 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca 901 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt 961 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta 1021 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct 1081 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca 1141 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta 1201 ctgtgtctcc atacccgttt ttttgggcta gcgtaggagg caaaaatgtg ggtcatcgag 1261 acaaccgaca cctttgatga gtggttcgat gctctggatg ataccgatag agcaaacgtg 1321 ctggcttcga tgatggtgct gcgagataga ggccccatgc tgtcgaggcc atacgcggat 1381 actgttaacg gttcatccta cagcaacatg aaagagcttc gggtccaaag caaaggagat 1441 cctatcagag cgttctttgc gttcgatcca aagcgtaagg ggattcttct ctgcgccggt 1501 aacaagaccg gggacgagaa aaggttttat gaagtaatga tcccaattgc agaccgtgag 1561 tttgcggcgc acttggataa attgaagaag gagtgtcgag ctcggtaccc ggggatcctc 1621 tagagtcgac ctgcaggcat gcaagcttgg ctgttttggc ggatgagaga agattttcag 1681 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg 1741 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc 1801 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac 1861 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 1921 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag 1981 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc 2041 tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca 2101 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 2161 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 2221 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 2281 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 2341 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 2401 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 2461 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 2521 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 2581 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 2641 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 2701 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 2761 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 2821 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 2881 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 2941 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 3001 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactc //