LOCUS       BBa_K1744002            3171 bp    DNA     linear       23-Jun-2024
DEFINITION  araC-PBAD-vcrx028-P1U8-amilCP-kanR
ACCESSION   BBa_K1744002
VERSION     BBa_K1744002.1  
FEATURES             Location/Qualifiers
     primer_bind     1..22
                     /label=forward priming site
     CDS             23..901
                     /label=araC
     misc_feature    930..947
                     /label=operator O2
     promoter        1052..1080
                     /label=araC promoter
     misc_feature    1088..1109
                     /label=operator O1
     misc_feature    1131..1144
                     /label=CAP site
     misc_feature    1140..1178
                     /label=operator I2 and I1
     promoter        1177..1204
                     /label=PBAD promoter
     misc_feature    1205..1205
                     /label=T > G
     misc_feature    1213..1213
                     /label=TSS
     RBS             1234..1245
                     /label=RBS
     CDS             1246..1623
                     /label=vcrx028
     misc_feature    1596..1596
                     /label=A > T, was the native stop codon of vcrx028
     promoter        1624..1658
                     /label=promoter-P1
     RBS             1663..1683
                     /label=UTR-U8
     CDS             1684..2352
                     /label=amilCP
     CDS             2560..3171
                     /label=kanR
     promoter        2353..2559
                     /label=kanR-promoter
ORIGIN
        1 caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt
       61 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
      121 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
      181 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
      241 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
      301 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
      361 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
      421 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
      481 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
      541 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
      601 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
      661 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
      721 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
      781 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
      841 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
      901 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt
      961 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta
     1021 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
     1081 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
     1141 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
     1201 ctgtgtctcc atacccgttt ttttgggcta gcgtaggagg caaaaatgtg ggtcatcgag
     1261 acaaccgaca cctttgatga gtggttcgat gctctggatg ataccgatag agcaaacgtg
     1321 ctggcttcga tgatggtgct gcgagataga ggccccatgc tgtcgaggcc atacgcggat
     1381 actgttaacg gttcatccta cagcaacatg aaagagcttc gggtccaaag caaaggagat
     1441 cctatcagag cgttctttgc gttcgatcca aagcgtaagg ggattcttct ctgcgccggt
     1501 aacaagaccg gggacgagaa aaggttttat gaagtaatga tcccaattgc agaccgtgag
     1561 tttgcggcgc acttggataa attgaagaag gagtgtcgag ctcggtaccc ggggatcctc
     1621 tagtttacag ctagctcagt cctaggtatt atgctagctt tgaaaattaa gaggtatata
     1681 ttaatgagtg tgatcgctaa acaaatgacc tacaaggttt atatgtcagg cacggtcaat
     1741 ggacactact ttgaggtcga aggcgatgga aaaggtaagc cctacgaggg ggagcagacg
     1801 gtaaagctca ctgtcaccaa gggcggacct ctgccatttg cttgggatat tttatcacca
     1861 cagtgtcagt acggaagcat accattcacc aagtaccctg aagacatccc tgactatgta
     1921 aagcagtcat tcccggaggg ctatacatgg gagaggatca tgaactttga agatggtgca
     1981 gtgtgtactg tcagcaatga ttccagcatc caaggcaact gtttcatcta ccatgtcaag
     2041 ttctctggtt tgaactttcc tcccaatgga cctgtcatgc agaagaagac acagggctgg
     2101 gaacccaaca ctgagcgtct ctttgcacga gatggaatgc tgctaggaaa caactttatg
     2161 gctctgaagt tagaaggagg cggtcactat ttgtgtgaat ttaaaactac ttacaaggca
     2221 aagaagcctg tgaagatgcc agggtatcac tatgttgacc gcaaactgga tgtaaccaat
     2281 cacaacaagg attacacttc ggttgagcag tgtgaaattt ccattgcacg caaacctgtg
     2341 gtcgcctaat aaatgagcca tattcaacgg gaaacgtctt gctcgtcgag gccgcgatta
     2401 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2461 tcaggtgcga caatctattg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2521 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2581 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2641 ttactcacca ctgcgatccc tgggaaaaca gcattccagg tattagaaga atatcctgat
     2701 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2761 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2821 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2881 gaacaagtct ggaaagaaat gcataacctt ttgccattct caccggattc agtcgtcact
     2941 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     3001 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3061 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3121 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta a
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