BBa_K1745001 1 KaiABC KaiABC Oscillator 2015-09-16T11:00:00Z 2015-09-18T12:10:38Z The KaiABC circadian oscillator is endogenous to the cynanobacterial species Synechococcus elongatus. pMC001 exhibits the three Kai proteins: KaiA, KaiB, and KaiC, which constitute a circadian oscillator. This biobrick can be used to drive circadian processes. Oscillations can be tracked through the phosphorylation states of KaiC. false false _2167_ 26239 26239 9 false In vitro investigations of this modular system revealed that the robustness of oscillations was sensitive to stoichiometric ratios of KaiA to KaiC (Nakajima, Science 2015). To experiment with these stoichiometric ratios, KaiB and KaiC expression are driven under a constitutive promoter (Part J23100) while KaiA expression is driven under a L-rhamnose inducible promoter (Part K914003). false Mansi Chandra annotation2467385 1 pRha Inducible BBa_K914003 range2467385 1 1 121 annotation2467387 1 Kai A range2467387 1 144 1001 annotation2467390 1 RBS BBa_B0034 range2467390 1 2658 2670 annotation2467386 1 RBS BBa_B0034 range2467386 1 127 138 annotation2467391 1 Kai B range2467391 1 2677 2983 annotation2467393 1 Constitutive BBa_J23100 range2467393 1 3013 3045 annotation2467389 1 Kai C range2467389 1 1091 2652 annotation2467388 1 Double Terminator BBa_B0014 range2467388 1 1002 1090 annotation2467392 1 RBS BBa_B0034 range2467392 1 2990 3006 BBa_K1745001_sequence 1 ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaaggtaccaaagaggagaaaggtaccgtgctctcgcaaattgcaatctgcatttgggtggaatcgacggcaattttgcaggattgccagcgggcgctgtcggccgatcgctatcaactccaagtctgtgagtctggcgaaatgctcttggagtatgcccaaacccatcgtgaccaaatcgactgcctgattttagtggcagccaatcccagcttcagggcagttgttcagcagctctgctttgagggagtggtggtaccagcgattgtcgtaggcgatcgcgacagtgaggatcccgatgaaccagccaaagaacagctctatcacagcgctgaactgcacctcggtatccatcagctcgagcaattgccctaccaagttgatgctgcactggctgaatttctgcgcttagccccggtcgagaccatggccgaccacatcatgctgatgggggccaaccacgatcccgagctatcgagccagcagcgggacctcgctcagcgactacaagagcgcctaggctatctcggggtctactacaagcgtgatcccgatcgctttctgcgcaacctacccgcctacgaaagccaaaagctgcaccaagcgatgcagactagctatcgtgaaatcgttttgagctatttttcgccgaatagcaacctcaaccagagcattgacaacttcgtcaacatggctttctttgccgatgttccagtcaccaaagtggtagaaattcacatggagctgatggacgagtttgccaagaagctccgcgtagagggacgttcagaggacattttgctggattatcggctgactttaattgatgtaattgcacatctttgtgagatgtatcgacggtctatcccacgagaaacctgatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttctagctctccggccctttttcttgaacgccgcgcacaattcgcgagagttcgcttttctcatcgacggtaatccgtgttggcgaacctgaaataatccgctcaaagttccggaaagaatccttgatgtccggccctttgtcgctgatcataaattcgcggattgctttgtcatgccaagatccgcgcatcttgaagacgttaatggcgcgggacatttcgccacgaatctcgacgtattggagcaagataatcgtatccgtaattgttgagatatgggagtcagtaatcgaatgcgctcccataaattgatcactggtatttgtgaatagtcccgtgatttcttcttgtttcgcgtagccagtgacaccaattacaaattggcggaaggcattgttgctaacgccccgcgccaaagcagagagggagtcgattgcaatacgagctggcttaaagtcattgatctccgacttaataatctgcaaatggtcttcaaggcctgcggattcaggataggcgcaaacaattttgaggaggttttggcgctccatctcctcaaagtccattccccatgaataggcattgcggagcagctgagctcgtgactcttcataagcgaacagaatcgcccgctctttgttagcacaagcattctcaacgaaacggctaactaacagagttttaccagtgcctgtagcgccagttgccagaatgattgagtccttaaagaagcccccaccacacatttcatcgagtcggacgacaccagatgaaacacgcacgttcgacgatcgctgcgtaaggcgcattgccccgagcgggaagatattgatgccatgatccgtaatcgtgaacggatattcccctttcatgtggctggtgccacgtagcttgaggatttcgagggtgcggcgacggcgctccccttccaaaacgttgcggagaatcacgacgttatcggagacaaattcctcaacaccgtaacgagcgatcgggccatattcctcgatacgctcggtggtcatgaccgtagttgccccaatttgttttaggcgagctaccaaccgaaagagttcgcggcgaaccacagaagaggcatcatattgctggaaaacggacgtgaccgagtcaattgaaacccgccgcgctcgatacttttgaattgcataattaatccgctcaatcagagcagagagatcgaagccgccaacaacctcttgaccttctggatcgggtgaagcatcaagaataaatagtttgccctcatcgaccagcttggctaaatcccagccaaaactacgggcgtttttaatgatatcttgcggggtttcttcgaaagtaacgaaaaccccaggctcatcaaactcgataataccgttatagagaaattgaatagaaaaaagggtcttgccggttcctgaagtaccactaacgagggtcgatcgcccgattggaagaccgccatgactaatatcatcaaagccttcaatcatcgtgcgcatcttagcgatggcttggtgctcagaattattattagggctagtcatctcagcggaagtcatggtacctttctcctctttggtaccttagaagtcgtcggaatcttgaagttcgccgtagagtaaatcaaggccaatcaaaactttctcacggtcggataaatcaccaataatccgtcggacaggcagtggtagaaccttggcgagggttggcgtcgctaggattttatcctcttctgccaactgaggatttttgagaacatcgatcacctttagagcataaacaccttgaaattcaacttcgagaatgttcttgagcgttttgagggcacggactgagtttggagtattgccggcgacgtagagcttgagaatgtaggttttacgagggctcatggtacctttctcctctttggtaccgctagcactgtacctaggactgagctagccgtcaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z