BBa_K1745002 1 BBa_K1745002 AANAT Expression 2015-09-16T11:00:00Z 2015-09-18T01:00:50Z Homo Sapiens (Human) Expression and possibly secretion of enzyme AANAT (aralkylamine N-acetyltransferase) [ Homo sapiens (human) ]. AANAT catalyzes Serotonin into N-acetylserotonin, a Melatonin precursor, and it is the rate-limiting step in Melatonin production. (Zheng 2002) Melatonin (N-acetyl-5-methoxytryptamine) is resposible for regulating circadium rhythms such as the sleep cycle and seasonal behaviors such as color change and reproduction in animals.(Lincoln 2003) In humans, AANAT is secreted in the Pineal gland. This construct is His-tagged. false false _2167_ 26241 26241 9 false One thing that was uncertain was whether the AANAT could be secreted after expression, and if it could be secreted, whether it could retain its activity or not. We had thought about adding secretion pathway signals such as the Tat and Sec signal to the construct, and we had also thought about using addition methods such as adding phage shock protein genes and Kil genes, but in the end we decided against it because it would be hard to assay. We resolved to put these other genes on separate constructs. false Benjamin Hsu annotation2472979 1 AANAT(human) range2472979 1 61 684 annotation2473562 1 RBS range2473562 1 37 61 annotation2473561 1 Constitutive Promoter range2473561 1 1 36 annotation2472978 1 His Tag range2472978 1 684 713 BBa_K1745002_sequence 1 ttgacggctagctcagtcctaggtacagtgctagcggtaccaaagaggagaaaggtaccatgtccatgcagagcacccaccccctgaaacctgaggccccacgtctgccacctgggatccccgagtccccgagctgtcagcggcgccacacactccctgccagtgagtttcgctgcctcaccccggaggacgctgtcagcgcctttgagatcgagcgtgaagccttcatctccgtcttgggcgtctgccccctgtacctggatgagatccggcacttcctgaccctatgtccagagctgtccctgggctggttcgaggagggctgccttgtggccttcatcatcggctcgctctgggacaaggagagactcatgcaggagtcactgacgctgcacaggtctgggggccacatagcccacctgcatgtgctggccgtgcaccgcgccttccggcagcagggcaggggccccatcctgctgtggcgctacctgcaccacctgggcagccagccggccgtgcgccgggccgcgctcatgtgcgaggacgcgctggtacccttctatgagaggttcagcttccacgccgtgggcccctgcgccatcaccgtgggctccctcaccttcatggagctccactgctccctgcggggccaccccttcctgcgcaggaacagcggctgccatcatcaccatcaccaccatcatcaccattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z