BBa_K1746000 1 BBa_K1746000 TdT delta1-27 (N-terminal Truncated Variant of TdT) 2015-09-10T11:00:00Z 2015-09-11T12:20:04Z This DNA sequence was chemically synthesized and codon optimized for expression in E. Coli. This DNA sequence is a truncation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. This deletion was of a putative nuclear localization sequence. This sequence was demonstrated to show 80% catalytic activity relative to full length, untruncated, TdT in 293T cells. false false _2168_ 27667 27667 9 false This DNA sequence was based on a truncated variant of bovine TdT which was then codon optimized for optimal expression in E. Coli, and chemically synthesized. false Tushar Nichakawade annotation2449392 1 Start Site range2449392 1 3 6 annotation2449391 1 NdeI cloning site range2449391 1 1 6 annotation2449393 1 Stop Site range2449393 1 1485 1491 annotation2449394 1 TdT delta1-27 range2449394 1 7 1484 BBa_K1746000_sequence 1 catatgcgccaagtgggtgcctctatggcctcaccaccgcatgatattaagttccagaacttagtcctgtttattctcgagaaaaaaatgggtaccacccgccgcaatttcctgatggaactggctcgccgtaagggttttcgcgttgagaacgaactgagcgacagcgtgacacacatcgtggcggagaataactccggctccgaagttttggagtggcttcaagttcagaacattcgcgcctcatcccagttagagctcttagatgtgtcctggctgattgaatcaatgggcgcgggcaaaccagtggagattacgggcaaacaccaactcgtagtgcgcacagattacagcgctaccccgaatcccggtttccagaaaaccccgccgttagcagtgaagaaaatttctcaatatgcctgccagcgtaaaacgaccttaaacaattataatcatattttcaccgatgcgttcgaaatcctcgccgaaaactctgaatttaaagaaaacgaagttagttatgttactttcatgcgtgccgccagtgttctgaaatcgctcccgttcaccattattagcatgaaagacactgagggtatcccatgtctgggggataaagttaagtgtattattgaagagatcattgaagatggcgagagcagcgaggtgaaagcggttttgaacgatgaacggtaccagagctttaaacttttcacaagtgtcttcggagtaggcctgaaaacctcggagaagtggtttcgcatgggttttcgttctctgtctaaaattatgagtgataaaaccctgaagtttaccaagatgcaaaaagccggatttttatactacgaagacctggtctcttgtgtaacccgtgccgaagcggaagcggtgggtgtgctggtgaaagaagctgtatgggcgtttctgccggacgccttcgttacaatgacgggcggattccgtcgtggtaagaaaattggacacgacgttgatttcctgattacgtcaccgggttcagctgaagatgaggaacagctgctcccgaaggtcattaacctgtgggagaaaaaaggcttactgctgtattatgacctggtggaaagcactttcgagaaatttaaactgccgagccgtcaggtggacacgctggatcacttccagaaatgcttcctgatcctgaaactgcatcatcagcgcgttgatagttcaaagtctaatcagcaggaaggcaaaacttggaaggcgattcgtgtggaccttgttatgtgtccttacgaaaatcgcgcgtttgctctgctggggtggaccggttcgcgccagttcgaacgtgatattcggcgttacgcgactcacgagcgtaaaatgatgttagacaaccatgctctgtacgataaaaccaaacgtgttttcctgaaagcagaatcggaggaagaaatttttgcccacctggggctggattatattgagccgtgggagcgtaatgcctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z