BBa_K1746001 1 BBa_K1746001 TdT delta26-143 (Truncated TdT Variant) 2015-09-10T11:00:00Z 2015-09-11T12:57:22Z This DNA sequence was chemically synthesized and codon optimized for expression in E. Coli. This DNA sequence is a mutated variant of a sequence coding for the bovine terminal deoxytransferase (TdT) enzyme. The sequence was originally desinged by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. It includes a deletion of the 26th to 143rd amino acids of the naturally occurring TdT protein. It shows 55% catalytic activity relative to full length TdT in 293T cells. It is intended to be used for adding nucleotides to the 3 prime end of a DNA sequence. false false _2168_ 27644 27644 9 false This DNA sequence was based on a truncated variant of bovine TdT which was then codon optimized for optimal expression in E. Coli, and chemically synthesized. false Christopher Lastihenos annotation2449414 1 stop codon range2449414 1 1212 1214 annotation2449411 1 Start Codon range2449411 1 4 6 annotation2449412 1 NdeI Site range2449412 1 1 6 annotation2449413 1 Protein coding sequence range2449413 1 3 1211 BBa_K1746001_sequence 1 catatggcacaacaacgtcagcatcagcgcctgccgatggacccgctgtgtactgcaagctccggtcctcgcaaaaaacggctaccccgaatcccggtttccagaaaaccccgccgttagcagtgaagaaaatttctcaatatgcctgccagcgtaaaacgaccttaaacaattataatcatattttcaccgatgcgttcgaaatcctcgccgaaaactctgaatttaaagaaaacgaagttagttatgttactttcatgcgtgccgccagtgttctgaaatcgctcccgttcaccattattagcatgaaagacactgagggtatcccatgtctgggggataaagttaagtgtattattgaagagatcattgaagatggcgagagcagcgaggtgaaagcggttttgaacgatgaacggtaccagagctttaaacttttcacaagtgtcttcggagtaggcctgaaaacctcggagaagtggtttcgcatgggttttcgttctctgtctaaaattatgagtgataaaaccctgaagtttaccaagatgcaaaaagccggatttttatactacgaagacctggtctcttgtgtaacccgtgccgaagcggaagcggtgggtgtgctggtgaaagaagctgtatgggcgtttctgccggacgccttcgttacaatgacgggcggattccgtcgtggtaagaaaattggacacgacgttgatttcctgattacgtcaccgggttcagctgaagatgaggaacagctgctcccgaaggtcattaacctgtgggagaaaaaaggcttactgctgtattatgacctggtggaaagcactttcgagaaatttaaactgccgagccgtcaggtggacacgctggatcacttccagaaatgcttcctgatcctgaaactgcatcatcagcgcgttgatagttcaaagtctaatcagcaggaaggcaaaacttggaaggcgattcgtgtggaccttgttatgtgtccttacgaaaatcgcgcgtttgctctgctggggtggaccggttcgcgccagttcgaacgtgatattcggcgttacgcgactcacgagcgtaaaatgatgttagacaaccatgctctgtacgataaaaccaaacgtgttttcctgaaagcagaatcggaggaagaaatttttgcccacctggggctggattatattgagccgtgggagcgtaatgcctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z