BBa_K1746002
1
BBa_K1746002
TdT GIP 213-215 subAAA (Mutated TdT Variant)
2015-09-10T11:00:00Z
2015-09-11T03:08:11Z
This DNA sequence was chemically synthesized and codon optimized for expression in E. Coli.
This DNA sequence is a mutation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. To create this sequence, amino acids 215-218 GIP, were substituted with AAA. This sequence was demonstrated to show 63% catalytic activity relative to full length, un-mutated, TdT. Expression of this sequence results in a protein that can be used for 3'-end non-templated synthesis of oligonucleotides.
false
false
_2168_
27667
27667
9
false
This DNA sequence was based on a truncated variant of bovine TdT which was then codon optimized for optimal expression in E. Coli, and chemically synthesized.
false
Tushar Nichakawade
annotation2449499
1
Stop
range2449499
1
1563
1569
annotation2449498
1
Start
range2449498
1
4
6
annotation2449497
1
NdeI
range2449497
1
1
6
annotation2449500
1
TdT GIP subAAA
range2449500
1
7
1562
BBa_K1746002_sequence
1
catatggcacaacaacgtcagcatcagcgcctgccgatggacccgctgtgtactgcaagctccggtcctcgcaaaaaacgtccgcgccaagtgggtgcctctatggcctcaccaccgcatgatattaagttccagaacttagtcctgtttattctcgagaaaaaaatgggtaccacccgccgcaatttcctgatggaactggctcgccgtaagggttttcgcgttgagaacgaactgagcgacagcgtgacacacatcgtggcggagaataactccggctccgaagttttggagtggcttcaagttcagaacattcgcgcctcatcccagttagagctcttagatgtgtcctggctgattgaatcaatgggcgcgggcaaaccagtggagattacgggcaaacaccaactcgtagtgcgcacagattacagcgctaccccgaatcccggtttccagaaaaccccgccgttagcagtgaagaaaatttctcaatatgcctgccagcgtaaaacgaccttaaacaattataatcatattttcaccgatgcgttcgaaatcctcgccgaaaactctgaatttaaagaaaacgaagttagttatgttactttcatgcgtgccgccagtgttctgaaatcgctcccgttcaccattattagcatgaaagacactgaggcggcggcgtgtctgggggataaagttaagtgtattattgaagagatcattgaagatggcgagagcagcgaggtgaaagcggttttgaacgatgaacggtaccagagctttaaacttttcacaagtgtcttcggagtaggcctgaaaacctcggagaagtggtttcgcatgggttttcgttctctgtctaaaattatgagtgataaaaccctgaagtttaccaagatgcaaaaagccggatttttatactacgaagacctggtctcttgtgtaacccgtgccgaagcggaagcggtgggtgtgctggtgaaagaagctgtatgggcgtttctgccggacgccttcgttacaatgacgggcggattccgtcgtggtaagaaaattggacacgacgttgatttcctgattacgtcaccgggttcagctgaagatgaggaacagctgctcccgaaggtcattaacctgtgggagaaaaaaggcttactgctgtattatgacctggtggaaagcactttcgagaaatttaaactgccgagccgtcaggtggacacgctggatcacttccagaaatgcttcctgatcctgaaactgcatcatcagcgcgttgatagttcaaagtctaatcagcaggaaggcaaaacttggaaggcgattcgtgtggaccttgttatgtgtccttacgaaaatcgcgcgtttgctctgctggggtggaccggttcgcgccagttcgaacgtgatattcggcgttacgcgactcacgagcgtaaaatgatgttagacaaccatgctctgtacgataaaaccaaacgtgttttcctgaaagcagaatcggaggaagaaatttttgcccacctggggctggattatattgagccgtgggagcgtaatgcctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z