BBa_K1746002 1 BBa_K1746002 TdT GIP 213-215 subAAA (Mutated TdT Variant) 2015-09-10T11:00:00Z 2015-09-11T03:08:11Z This DNA sequence was chemically synthesized and codon optimized for expression in E. Coli. This DNA sequence is a mutation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. To create this sequence, amino acids 215-218 GIP, were substituted with AAA. This sequence was demonstrated to show 63% catalytic activity relative to full length, un-mutated, TdT. Expression of this sequence results in a protein that can be used for 3'-end non-templated synthesis of oligonucleotides. false false _2168_ 27667 27667 9 false This DNA sequence was based on a truncated variant of bovine TdT which was then codon optimized for optimal expression in E. Coli, and chemically synthesized. false Tushar Nichakawade annotation2449499 1 Stop range2449499 1 1563 1569 annotation2449498 1 Start range2449498 1 4 6 annotation2449497 1 NdeI range2449497 1 1 6 annotation2449500 1 TdT GIP subAAA range2449500 1 7 1562 BBa_K1746002_sequence 1 catatggcacaacaacgtcagcatcagcgcctgccgatggacccgctgtgtactgcaagctccggtcctcgcaaaaaacgtccgcgccaagtgggtgcctctatggcctcaccaccgcatgatattaagttccagaacttagtcctgtttattctcgagaaaaaaatgggtaccacccgccgcaatttcctgatggaactggctcgccgtaagggttttcgcgttgagaacgaactgagcgacagcgtgacacacatcgtggcggagaataactccggctccgaagttttggagtggcttcaagttcagaacattcgcgcctcatcccagttagagctcttagatgtgtcctggctgattgaatcaatgggcgcgggcaaaccagtggagattacgggcaaacaccaactcgtagtgcgcacagattacagcgctaccccgaatcccggtttccagaaaaccccgccgttagcagtgaagaaaatttctcaatatgcctgccagcgtaaaacgaccttaaacaattataatcatattttcaccgatgcgttcgaaatcctcgccgaaaactctgaatttaaagaaaacgaagttagttatgttactttcatgcgtgccgccagtgttctgaaatcgctcccgttcaccattattagcatgaaagacactgaggcggcggcgtgtctgggggataaagttaagtgtattattgaagagatcattgaagatggcgagagcagcgaggtgaaagcggttttgaacgatgaacggtaccagagctttaaacttttcacaagtgtcttcggagtaggcctgaaaacctcggagaagtggtttcgcatgggttttcgttctctgtctaaaattatgagtgataaaaccctgaagtttaccaagatgcaaaaagccggatttttatactacgaagacctggtctcttgtgtaacccgtgccgaagcggaagcggtgggtgtgctggtgaaagaagctgtatgggcgtttctgccggacgccttcgttacaatgacgggcggattccgtcgtggtaagaaaattggacacgacgttgatttcctgattacgtcaccgggttcagctgaagatgaggaacagctgctcccgaaggtcattaacctgtgggagaaaaaaggcttactgctgtattatgacctggtggaaagcactttcgagaaatttaaactgccgagccgtcaggtggacacgctggatcacttccagaaatgcttcctgatcctgaaactgcatcatcagcgcgttgatagttcaaagtctaatcagcaggaaggcaaaacttggaaggcgattcgtgtggaccttgttatgtgtccttacgaaaatcgcgcgtttgctctgctggggtggaccggttcgcgccagttcgaacgtgatattcggcgttacgcgactcacgagcgtaaaatgatgttagacaaccatgctctgtacgataaaaccaaacgtgttttcctgaaagcagaatcggaggaagaaatttttgcccacctggggctggattatattgagccgtgggagcgtaatgcctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z