##gff-version 3
##sequence-region BBa_K1747012 1 7156
BBa_K1747012	.	terminator	7028	7156	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1747012	.	terminator	7116	7156	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1747012	.	stem_loop	7123	7142	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1747012	.	polyA_site	7143	7156	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1747012	.	stop_codon	7149	7149	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1747012	.	engineered_region	7116	7156	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1747012	.	terminator	7028	7107	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1747012	.	stem_loop	7039	7082	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1747012	.	engineered_region	7028	7107	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1747012	.	engineered_region	6074	7019	.	+	0	ID=BBa_K817007;Name=BBa_K817007
BBa_K1747012	.	engineered_region	6074	6293	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K817007
BBa_K1747012	.	promoter	6074	6273	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1747012	.	CDS	6074	6161	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1747012	.	promoter	6234	6239	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1747012	.	non_covalent_binding_site	6239	6273	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1747012	.	engineered_region	6074	6273	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1747012	.	promoter	6210	6215	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1747012	.	non_covalent_binding_site	6162	6199	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1747012	.	start_codon	6246	6246	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1747012	.	ribosome_entry_site	6282	6293	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1747012	.	conserved_region	6286	6289	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1747012	.	CDS	6300	7019	.	+	0	ID=BBa_K817001;Name=BBa_K817001;Parent=BBa_K817007
BBa_K1747012	.	CDS	6300	7019	.	+	0	ID=annotation2187738;Name=FadR;Parent=BBa_K817001
BBa_K1747012	.	engineered_region	1	6065	.	+	0	ID=BBa_K1747011;Name=BBa_K1747011
BBa_K1747012	.	CDS	1384	2295	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K1747011
BBa_K1747012	.	engineered_region	819	1365	.	+	0	ID=BBa_I742158;Name=BBa_I742158;Parent=BBa_K1747011
BBa_K1747012	.	ribosome_entry_site	819	834	.	+	0	ID=BBa_I742156;Name=BBa_I742156;Parent=BBa_I742158
BBa_K1747012	.	CDS	835	1365	.	+	0	ID=BBa_I742157;Name=BBa_I742157;Parent=BBa_I742158
BBa_K1747012	.	CDS	835	1362	.	+	0	ID=annotation1953812;Name=crtZ;Parent=BBa_I742157
BBa_K1747012	.	ribosome_entry_site	4767	4770	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K1747011
BBa_K1747012	.	CDS	4777	5928	.	+	0	ID=BBa_K118008;Name=BBa_K118008;Parent=BBa_K1747011
BBa_K1747012	.	CDS	4777	5925	.	+	0	ID=annotation1995716;Name=crtY coding sequence;Parent=BBa_K118008
BBa_K1747012	.	terminator	5937	6065	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1747011
BBa_K1747012	.	terminator	6025	6065	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1747012	.	stem_loop	6032	6051	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1747012	.	polyA_site	6052	6065	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1747012	.	stop_codon	6058	6058	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1747012	.	engineered_region	6025	6065	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1747012	.	terminator	5937	6016	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1747012	.	stem_loop	5948	5991	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1747012	.	engineered_region	5937	6016	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1747012	.	CDS	2320	3252	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K1747011
BBa_K1747012	.	CDS	2320	3249	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_K1747012	.	engineered_region	1	84	.	+	0	ID=BBa_K1747004;Name=BBa_K1747004;Parent=BBa_K1747011
BBa_K1747012	.	ribosome_entry_site	73	84	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1747004
BBa_K1747012	.	conserved_region	77	80	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1747012	.	promoter	1	72	.	+	0	ID=BBa_K817002;Name=BBa_K817002;Parent=BBa_K1747004
BBa_K1747012	.	promoter	1	72	.	+	0	ID=annotation2197686;Name=PfadBA;Parent=BBa_K817002
BBa_K1747012	.	ribosome_entry_site	2304	2313	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K1747011
BBa_K1747012	.	sequence_feature	2304	2306	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K1747012	.	ribosome_entry_site	2307	2313	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K1747012	.	ribosome_entry_site	3261	3270	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K1747011
BBa_K1747012	.	sequence_feature	3261	3263	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K1747012	.	ribosome_entry_site	3264	3270	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K1747012	.	CDS	91	810	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K1747011
BBa_K1747012	.	CDS	91	810	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1747012	.	ribosome_entry_site	1374	1377	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K1747011
BBa_K1747012	.	CDS	3277	4758	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K1747011
BBa_K1747012	.	point_mutation	4165	4170	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_K1747012	.	point_mutation	3754	3759	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_K1747012	.	CDS	3277	4755	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
>BBa_K1747012
atctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgaccaaagagg
agaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaa
tgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcact
actggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccag
atcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaaga
tgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtatt
gattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacca
ttatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctt
tcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg
atgaactatacaaataataatactagagctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgt
taccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctt
tcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgc
tgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattt
tatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtat
atggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgt
caaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatga
gcccgcatccgggaagtaataatactagaggaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgc
gatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttg
tgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcga
tctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatc
cttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatg
tggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggc
aaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtct
gaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctcca
tgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtca
ggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcg
acgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctct
ctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagtta
ataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggc
tcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgcc
gccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacg
tctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcag
gaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaag
cgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaat
catgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgc
gatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagaga
attatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactattt
gtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaa
ataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgc
tgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcg
cccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctg
gcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggctt
atgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtt
tgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtca
gggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaag
gttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatc
gttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagt
tacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcat
ccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttca
ggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacagga
aacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttc
atacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaa
ctctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgt
taccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtg
tcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaa
cctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggctta
cggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagcct
tttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacct
ggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctg
gaggatctgatataataatactagaggaggtactagatgcaaccgcattatgatctgattctcgtgggggctggactcg
cgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggc
gggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggtt
catcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctc
agcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcgga
atctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagca
ctgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatca
tggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattga
agacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacag
ggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattct
ggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggc
ggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcc
cgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgct
ggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccga
tcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaa
accgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgag
cgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgt
gtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggtcattaaggcgcaaagc
ccggcgggtttcgcggaagagtacattattgaaagtatctggaataaccgcttccctcccgggactattttgcccgcag
aacgtgaactttcagaattaattggcgtaacgcgtactacgttacgtgaagtgttacagcgtctggcacgagatggctg
gttgaccattcaacatggcaagccgacgaaggtgaataatttctgggaaacttccggtttaaatatccttgaaacactg
gcgcgactggatcacgaaagtgtgccgcagcttattgataatttgctgtcggtgcgtaccaatatttccactattttta
ttcgcaccgcgtttcgtcagcatcccgataaagcgcaggaagtgctggctaccgctaatgaagtggccgatcacgccga
tgcctttgccgagctggattacaacatattccgcggcctggcgtttgcttccggcaacccgatttacggtctgattctt
aacgggatgaaagggctgtatacgcgtattggtcgtcactatttcgccaatccggaagcgcgcagtctggcgctgggct
tctaccacaaactgtcggcgttgtgcagtgaaggcgcgcacgatcaggtgtacgaaacagtgcgtcgctatgggcatga
gagtggcgagatttggcaccggatgcagaaaaatctgccgggtgatttagccattcaggggcgataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata