LOCUS       BBa_K1749000            1440 bp    DNA     linear       25-Nov-2024
DEFINITION  Cd sensitive promoter with Phi delta activator, PO promoter, and 
            GFP
ACCESSION   BBa_K1749000
VERSION     BBa_K1749000.1  
FEATURES             Location/Qualifiers
     misc_feature    9..26
                     /label=ArsR binding site
     promoter        1..26
                     /label=BBa_K174016
     promoter        45..178
                     /label=cadA promoter
     misc_feature    52..57
                     /label=sigA -35
     misc_feature    76..81
                     /label=sigA -10
     misc_feature    90..120
                     /label=CzrA binding site
     promoter        35..205
                     /label=BBa_K174017
     misc_feature    214..225
                     /label=B0034
     CDS             235..477
                     /label=phiR73 delta
     misc_feature    235..235
                     /label=phiR73 delta
     misc_feature    315..315
                     /label=silent mutation to remove PstI site
     misc_feature    214..477
                     /label=BBa_I746352
     promoter        486..577
                     /label=PO
     promoter        486..577
                     /label=BBa_I746361
     CDS             584..1303
                     /label=GFP protein
     CDS             584..1303
                     /label=BBa_E0040
     stem_loop       1323..1366
                     /label=stem_loop
     misc_feature    1312..1391
                     /label=BBa_B0010
     terminator      1312..1391
                     /label=BBa_B0010
     stem_loop       1407..1426
                     /label=T7 TE
     misc_feature    1433..1433
                     /label=stop
     polyA_site      1427..1440
                     /label=polya
     misc_feature    1400..1440
                     /label=BBa_B0012
     terminator      1400..1440
                     /label=BBa_B0012
ORIGIN
        1 agtaatcaaa ataaattgat ttattttact agaggcgctt gctgtttttc attgacactt
       61 tcttggaaaa caacatataa taggtgtaac ttatatatga gtatatgctc atatatataa
      121 aataaataca atactcattg atacgctttg aagagggaac cctaaatcgg aaggtaagct
      181 aagaggagga actactatgg ctagctacta gagaaagagg agaaatacta gatgatgcgc
      241 tgccctttct gtcgtcattc agcgcatacc cgcaccagcc ggtatgtgag tgacaatgtc
      301 aaagaaagtt atctccagtg ccagaatatt tactgttcgg cgacatttaa aacgcatgag
      361 tcaatttgtg ccgtgattcg ttctccggtc acggaggaaa aaccagcacc ggcaagcaca
      421 gcaccggctg ttgtccgaaa agttaaaggc tgttacagct caccattcaa ccattaatac
      481 tagagcgcgc cccgcgattc gctaaggtgc tgttgtgtca gtgataagcc atccgggact
      541 gatggcggag gatgcgcatc gtcgggaaac tgatgcctac tagatgcgta aaggagaaga
      601 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      661 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      721 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
      781 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
      841 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
      901 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
      961 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
     1021 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     1081 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1141 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1201 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     1261 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc
     1321 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1381 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//