BBa_K1749000
type: ComponentDefinition
persistent identity: BBa K1749000
display id: BBa_K1749000
version: 1
was generated by: iGEM to SBOL conversion
description: Cd sensitive promoter with Phi delta activator, PO promoter, and GFP
created: 2015-09-16T11:00:00Z
modified: 2016-01-11T01:35:16Z
mutable provenance: This composite part contains only other biobrick parts.
owned by: james
owned by: myers
mutable description: The Cadmium sensitive promoter is combined with amplifiers from the 2009 Cambridge iGEM team to increase the sensitivity to cadmium. It uses GFP as a reporter.
discontinued: false
dominant: false
group u list: _2171_
m user id: 4206
owner id: 4261
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Anne Byford
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_I746361
component: BBa_I746352
component: BBa_B0010
component: BBa_E0040
component: BBa_K174017
component: BBa_K174016
sequence annotation: BBa_K174016
sequence annotation: BBa_K174017
sequence annotation: BBa_I746352
sequence annotation: BBa_I746361
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1749000_sequence/1

BBa_B0012
type: Component
persistent identity: component2466673
display id: component2466673
version: 1
top level: BBa_K1749000
definition: BBa_B0012
access: public

BBa_I746361
type: Component
persistent identity: component2466668
display id: component2466668
version: 1
top level: BBa_K1749000
definition: BBa_I746361
access: public

BBa_I746352
type: Component
persistent identity: component2466666
display id: component2466666
version: 1
top level: BBa_K1749000
definition: BBa_I746352
access: public

BBa_B0010
type: Component
persistent identity: component2466671
display id: component2466671
version: 1
top level: BBa_K1749000
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component2466670
display id: component2466670
version: 1
top level: BBa_K1749000
definition: GFP
access: public

BBa_K174017
type: Component
persistent identity: component2466661
display id: component2466661
version: 1
top level: BBa_K1749000
definition: BBa_K174017
access: public

BBa_K174016
type: Component
persistent identity: component2466656
display id: component2466656
version: 1
top level: BBa_K1749000
definition: BBa_K174016
access: public

BBa_K174016
type: SequenceAnnotation
persistent identity: annotation2466656
display id: annotation2466656
version: 1
top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466656/range2466656/1
component: BBa_K174016

https://synbiohub.org/public/igem/BBa_K1749000/annotation2466656/range2466656/1
type: Range
persistent identity: range2466656
display id: range2466656
version: 1
top level: BBa_K1749000
direction: direction/reverse
start: 1
end: 26
orientation: inline

BBa_K174017
type: SequenceAnnotation
persistent identity: annotation2466661
display id: annotation2466661
version: 1
top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466661/range2466661/1
component: BBa_K174017

https://synbiohub.org/public/igem/BBa_K1749000/annotation2466661/range2466661/1
type: Range
persistent identity: range2466661
display id: range2466661
version: 1
top level: BBa_K1749000
direction: direction/reverse
start: 35
end: 205
orientation: inline

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2466666
display id: annotation2466666
version: 1
top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466666/range2466666/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_K1749000/annotation2466666/range2466666/1
type: Range
persistent identity: range2466666
display id: range2466666
version: 1
top level: BBa_K1749000
direction: direction/reverse
start: 214
end: 477
orientation: inline

BBa_I746361
type: SequenceAnnotation
persistent identity: annotation2466668
display id: annotation2466668
version: 1
top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466668/range2466668/1
component: BBa_I746361

https://synbiohub.org/public/igem/BBa_K1749000/annotation2466668/range2466668/1
type: Range
persistent identity: range2466668
display id: range2466668
version: 1
top level: BBa_K1749000
direction: direction/reverse
start: 486
end: 577
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2466670
display id: annotation2466670
version: 1
top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466670/range2466670/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1749000/annotation2466670/range2466670/1
type: Range
persistent identity: range2466670
display id: range2466670
version: 1
top level: BBa_K1749000
direction: direction/reverse
start: 584
end: 1303
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2466671
display id: annotation2466671
version: 1
top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466671/range2466671/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1749000/annotation2466671/range2466671/1
type: Range
persistent identity: range2466671
display id: range2466671
version: 1
top level: BBa_K1749000
direction: direction/forward
start: 1312
end: 1391
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2466673
display id: annotation2466673
version: 1
top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466673/range2466673/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1749000/annotation2466673/range2466673/1
type: Range
persistent identity: range2466673
display id: range2466673
version: 1
top level: BBa_K1749000
direction: direction/forward
start: 1400
end: 1440
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K174016
type: ComponentDefinition
persistent identity: BBa K174016
display id: BBa_K174016
version: 1
was generated by: iGEM to SBOL conversion
description: Promoterless ArsR binding site
created: 2009-10-13T11:00:00Z
modified: 2015-07-24T01:17:49Z
mutable provenance: ''B. subtilis''
owned by: james
owned by: myers
mutable description: This part purely holds the binding site for ArsR protein which can be released when bound to different metals. Hence this part can be combined with any promoter to sense metals that can bind to ArsR protein. When added in front of a promoter with another metal sensor's protein's binding site, it can form an AND gate to sensitively detect specific metals.
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 4206
owner id: 3942
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It was developed mainly to use combinatorial approaches for metal sensing.
star: false
creator: The Newcastle 2009 iGEM team
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ArsR binding site
sequence: https://synbiohub.org/public/igem/BBa_K174016_sequence/1

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation2040774
display id: annotation2040774
version: 1
top level: BBa_K174016
location: https://synbiohub.org/public/igem/BBa_K174016/annotation2040774/range2040774/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K174016/annotation2040774/range2040774/1
type: Range
persistent identity: range2040774
display id: range2040774
version: 1
top level: BBa_K174016
direction: direction/forward
start: 9
end: 26
orientation: inline

BBa_K174017
type: ComponentDefinition
persistent identity: BBa K174017
display id: BBa_K174017
version: 1
was generated by: iGEM to SBOL conversion
description: CadA promoter with CzrA binding site
created: 2009-10-13T11:00:00Z
modified: 2015-07-24T01:17:17Z
mutable provenance: It is taken from ''B. subtilis'' ''cadA'' promoter.
owned by: james
owned by: myers
mutable description: In ''B. subtilis'' CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by CzrA repressor protein. When certain metals get inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system. By taking the promoter of CadA system, we created a generic metal sensor. Hence when it is combined with coding sequences of other genes, it will regulate the expression of these genes upon sensing metals.
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 4206
owner id: 3942
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It was developed mainly to use combinatorial approaches for metal sensing
star: false
creator: The Newcastle 2009 iGEM team
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cadA promoter
sequence annotation: sigA -35
sequence annotation: sigA -10
sequence annotation: CzrA binding site
sequence: https://synbiohub.org/public/igem/BBa_K174017_sequence/1

cadA promoter
type: SequenceAnnotation
persistent identity: annotation2040827
display id: annotation2040827
version: 1
top level: BBa_K174017
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040827/range2040827/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K174017/annotation2040827/range2040827/1
type: Range
persistent identity: range2040827
display id: range2040827
version: 1
top level: BBa_K174017
direction: direction/forward
start: 11
end: 144
orientation: inline

sigA -35
type: SequenceAnnotation
persistent identity: annotation2040828
display id: annotation2040828
version: 1
top level: BBa_K174017
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040828/range2040828/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K174017/annotation2040828/range2040828/1
type: Range
persistent identity: range2040828
display id: range2040828
version: 1
top level: BBa_K174017
direction: direction/forward
start: 18
end: 23
orientation: inline

sigA -10
type: SequenceAnnotation
persistent identity: annotation2040829
display id: annotation2040829
version: 1
top level: BBa_K174017
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040829/range2040829/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K174017/annotation2040829/range2040829/1
type: Range
persistent identity: range2040829
display id: range2040829
version: 1
top level: BBa_K174017
direction: direction/forward
start: 42
end: 47
orientation: inline

CzrA binding site
type: SequenceAnnotation
persistent identity: annotation2040830
display id: annotation2040830
version: 1
top level: BBa_K174017
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040830/range2040830/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K174017/annotation2040830/range2040830/1
type: Range
persistent identity: range2040830
display id: range2040830
version: 1
top level: BBa_K174017
direction: direction/forward
start: 56
end: 86
orientation: inline

BBa_I746361
type: ComponentDefinition
persistent identity: BBa I746361
display id: BBa_I746361
version: 1
was generated by: iGEM to SBOL conversion
description: PO promoter from P2 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The source of the DNA is the P2 phage genome.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PO
sequence: https://synbiohub.org/public/igem/BBa_I746361_sequence/1

PO
type: SequenceAnnotation
persistent identity: annotation1943784
display id: annotation1943784
version: 1
top level: BBa_I746361
location: https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
type: Range
persistent identity: range1943784
display id: range1943784
version: 1
top level: BBa_I746361
direction: direction/forward
start: 1
end: 92
orientation: inline

BBa_I746352
type: ComponentDefinition
persistent identity: BBa I746352
display id: BBa_I746352
version: 1
was generated by: iGEM to SBOL conversion
description: delta activator from phiR73 phage
created: 2007-09-11T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
mutable description: The delta activator taken from phiR73 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The part does contain a RBS (B0034) already.
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: phiR73 delta
sequence annotation: silent mutation to remove PstI site
sequence: https://synbiohub.org/public/igem/BBa_I746352_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1943871
display id: annotation1943871
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
type: Range
persistent identity: range1943871
display id: range1943871
version: 1
top level: BBa_I746352
direction: direction/forward
start: 1
end: 12
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943872
display id: annotation1943872
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
type: Range
persistent identity: range1943872
display id: range1943872
version: 1
top level: BBa_I746352
direction: direction/forward
start: 22
end: 264
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943873
display id: annotation1943873
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
type: Range
persistent identity: range1943873
display id: range1943873
version: 1
top level: BBa_I746352
direction: direction/forward
start: 22
end: 22
orientation: inline

silent mutation to remove PstI site
type: SequenceAnnotation
persistent identity: annotation1943874
display id: annotation1943874
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
type: Range
persistent identity: range1943874
display id: range1943874
version: 1
top level: BBa_I746352
direction: direction/forward
start: 102
end: 102
orientation: inline

https://synbiohub.org/public/igem/BBa_K1749000_sequence/1
type: Sequence
persistent identity: BBa K1749000 sequence
display id: BBa_K1749000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtaatcaaaataaattgatttattttactagaggcgcttgctgtttttcattgacactttcttggaaaacaacatataataggtgtaacttatatatgagtatatgctcatatatataaaataaatacaatactcattgatacgctttgaagagggaaccctaaatcggaaggtaagctaagaggaggaactactatggctagctactagagaaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagcgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K174016_sequence/1
type: Sequence
persistent identity: BBa K174016 sequence
display id: BBa_K174016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtaatcaaaataaattgatttattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K174017_sequence/1
type: Sequence
persistent identity: BBa K174017 sequence
display id: BBa_K174017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgcttgctgtttttcattgacactttcttggaaaacaacatataataggtgtaacttatatatgagtatatgctcatatatataaaataaatacaatactcattgatacgctttgaagagggaaccctaaatcggaaggtaagctaagaggaggaactactatggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746361_sequence/1
type: Sequence
persistent identity: BBa I746361 sequence
display id: BBa_I746361_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746352_sequence/1
type: Sequence
persistent identity: BBa I746352 sequence
display id: BBa_I746352_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z