LOCUS       BBa_K175006             1483 bp    DNA     linear       22-Nov-2024
DEFINITION  [J13002][K175001][E0840][I714031] - constitutive trbK GFP and 
            oriT-R
ACCESSION   BBa_K175006
VERSION     BBa_K175006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..311
                     /label=trbK
     CDS             81..311
                     /label=BBa_K175001
     RBS             320..334
                     /label=RBS-1\Strong
     RBS             327..331
                     /label=RBS
     misc_feature    320..334
                     /label=BBa_B0030
     RBS             320..334
                     /label=BBa_B0030
     CDS             341..1060
                     /label=GFP protein
     CDS             341..1060
                     /label=BBa_E0040
     stem_loop       1080..1123
                     /label=stem_loop
     misc_feature    1069..1148
                     /label=BBa_B0010
     terminator      1069..1148
                     /label=BBa_B0010
     stem_loop       1164..1183
                     /label=T7 TE
     misc_feature    1190..1190
                     /label=stop
     polyA_site      1184..1197
                     /label=polya
     misc_feature    1157..1197
                     /label=BBa_B0012
     terminator      1157..1197
                     /label=BBa_B0012
     oriT            1206..1483
                     /label=BBa_I714031
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaagcga tcaaagcgct gtctctggcg tctgcggcgc
      121 tggttgcggc gctggttgcg ggttgcgaca acaaaccggc gaccgcgccg atgccggaag
      181 ttaacgacga atcttgcaaa ccggaaaaca tcgcgaaaat cgaagacaaa ggtgttcagc
      241 aggcgttctc ttctctgtgc ctgcgtcgtg gtggtgactt caaaccgtct ccgaaacgtg
      301 aatggtaata atactagaga ttaaagagga gaaatactag atgcgtaaag gagaagaact
      361 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      421 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      481 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
      541 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      601 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      661 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      721 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      781 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
      841 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      901 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
      961 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     1021 tgggattaca catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa
     1081 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1141 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     1201 tagaggatct cgtctttctc ttcggggaac accggcaccc gcaggtgctg ccgccgccgg
     1261 cggccgtgtt ccttttcgtc gttctccatg cctcgcctcg tctctcatgc cggcggtagc
     1321 cggctgcctc gcagagcagg atgacccgtt gagcgccccc ggcgcgaata agggacagtg
     1381 aagatagata accggctcgc cggttagcta acttcacaca tcctgcccgc cttacggcgt
     1441 taataacacc aaggaaagtc tacaccagcc attacgattt atc
//