##gff-version 3
##sequence-region BBa_K175006 1 1483
BBa_K175006	.	terminator	1069	1148	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K175006	.	engineered_region	1069	1148	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K175006	.	stem_loop	1080	1123	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175006	.	ribosome_entry_site	320	334	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K175006	.	engineered_region	320	334	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K175006	.	ribosome_entry_site	327	331	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K175006	.	ribosome_entry_site	320	334	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K175006	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K175006	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K175006	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K175006	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K175006	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K175006	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K175006	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K175006	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K175006	.	oriT	1206	1483	.	+	0	ID=BBa_I714031;Name=BBa_I714031
BBa_K175006	.	CDS	81	311	.	+	0	ID=BBa_K175001;Name=BBa_K175001
BBa_K175006	.	CDS	81	311	.	+	0	ID=annotation2009448;Name=trbK;Parent=BBa_K175001
BBa_K175006	.	terminator	1157	1197	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K175006	.	polyA_site	1184	1197	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175006	.	engineered_region	1157	1197	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175006	.	stop_codon	1190	1190	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175006	.	stem_loop	1164	1183	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175006	.	CDS	341	1060	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K175006	.	CDS	341	1060	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K175006
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaagcgatcaaagcgctgtctctggcgtctgcggcgctggttgcggcgctggttgcgggttgcgacaacaaaccg
gcgaccgcgccgatgccggaagttaacgacgaatcttgcaaaccggaaaacatcgcgaaaatcgaagacaaaggtgttc
agcaggcgttctcttctctgtgcctgcgtcgtggtggtgacttcaaaccgtctccgaaacgtgaatggtaataatacta
gagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattag
atggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaa
atttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgcttt
gcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaacta
tatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgta
tacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttc
aactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccac
acaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatt
acacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagaggatctcgtctttctcttcggggaacaccggcacccgcaggtgctgccgccgccggcggc
cgtgttccttttcgtcgttctccatgcctcgcctcgtctctcatgccggcggtagccggctgcctcgcagagcaggatg
acccgttgagcgcccccggcgcgaataagggacagtgaagatagataaccggctcgccggttagctaacttcacacatc
ctgcccgccttacggcgttaataacaccaaggaaagtctacaccagccattacgatttatc