##gff-version 3
##sequence-region BBa_K175020 1 1048
BBa_K175020	.	engineered_region	1	220	.	+	0	ID=BBa_K175016;Name=BBa_K175016
BBa_K175020	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K175016
BBa_K175020	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K175020	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K175016
BBa_K175020	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K175020	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K175020	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K175020	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K175020	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K175020	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K175020	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K175020	.	engineered_region	227	1048	.	+	0	ID=BBa_K175017;Name=BBa_K175017
BBa_K175020	.	CDS	227	911	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K175017
BBa_K175020	.	engineered_tag	887	911	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K175020	.	CDS	230	846	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K175020	.	tag	847	880	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K175020	.	terminator	920	1048	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K175017
BBa_K175020	.	terminator	1008	1048	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K175020	.	polyA_site	1035	1048	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175020	.	stop_codon	1041	1041	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175020	.	engineered_region	1008	1048	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175020	.	stem_loop	1015	1034	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175020	.	terminator	920	999	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K175020	.	stem_loop	931	974	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175020	.	engineered_region	920	999	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K175020
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtccagatt
agataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactc
gcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcca
ttgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgc
taaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacag
tatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcg
ctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctac
tactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattc
ggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacg
ctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata