LOCUS       BBa_K175021             1730 bp    DNA     linear       22-Dec-2024
DEFINITION  PTet+RBS+cI+RBS+RFP+T
ACCESSION   BBa_K175021
VERSION     BBa_K175021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             87..794
                     /label=cI lambda
     misc_feature    795..827
                     /label=LVA
     misc_feature    834..858
                     /label=Help:Barcodes
     CDS             84..858
                     /label=BBa_C0051
     RBS             867..881
                     /label=RBS-1\Strong
     RBS             874..878
                     /label=RBS
     misc_feature    867..881
                     /label=BBa_B0030
     RBS             867..881
                     /label=BBa_B0030
     misc_feature    63..881
                     /label=BBa_K081013
     misc_feature    1..881
                     /label=BBa_K175009
     CDS             888..1562
                     /label=mrfp1
     misc_feature    1569..1593
                     /label=Help:Barcodes
     CDS             888..1593
                     /label=BBa_E1010
     stem_loop       1613..1656
                     /label=stem_loop
     misc_feature    1602..1681
                     /label=BBa_B0010
     terminator      1602..1681
                     /label=BBa_B0010
     stem_loop       1697..1716
                     /label=T7 TE
     misc_feature    1723..1723
                     /label=stop
     polyA_site      1717..1730
                     /label=polya
     misc_feature    1690..1730
                     /label=BBa_B0012
     terminator      1690..1730
                     /label=BBa_B0012
     terminator      1602..1730
                     /label=BBa_B0015
     misc_feature    888..1730
                     /label=BBa_K175015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgagca caaaaaagaa accattaaca caagagcagc
      121 ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat
      181 cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt gctttattta
      241 atggcatcaa tgcattaaat gcttataacg ccgcattgct tgcaaaaatt ctcaaagtta
      301 gcgttgaaga atttagccct tcaatcgcca gagaaatcta cgagatgtat gaagcggtta
      361 gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat gttcaggcag
      421 ggatgttctc acctgagctt agaaccttta ccaaaggtga tgcggagaga tgggtaagca
      481 caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat tccatgaccg
      541 caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt gaccctgagc
      601 aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag tttaccttca
      661 agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca cagtacccaa
      721 tgatcccatg caatgagagt tgttccgttg tggggaaagt tatcgctagt cagtggcctg
      781 aagagacgtt tggcgctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat
      841 agtgctagtg tagatcgcta ctagagatta aagaggagaa atactagatg gcttcctccg
      901 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc
      961 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta
     1021 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt
     1081 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac
     1141 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg
     1201 ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc
     1261 gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag
     1321 cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc
     1381 tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa
     1441 aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca
     1501 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg
     1561 cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg
     1621 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1681 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//