LOCUS BBa_K175022 967 bp DNA linear 16-Dec-2024 DEFINITION PTet+Lock3c+GFP+TT ACCESSION BBa_K175022 VERSION BBa_K175022.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 63..104 /label=BBa_J23031 CDS 111..830 /label=GFP protein CDS 111..830 /label=BBa_E0040 stem_loop 850..893 /label=stem_loop misc_feature 839..918 /label=BBa_B0010 terminator 839..918 /label=BBa_B0010 stem_loop 934..953 /label=T7 TE misc_feature 960..960 /label=stop polyA_site 954..967 /label=polya misc_feature 927..967 /label=BBa_B0012 terminator 927..967 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaactagaa tcacctcttg cttttgggta agacgaagag gagatactag atgcgtaaag 121 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg 181 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc 241 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt 301 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt 361 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg 421 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 481 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca 541 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta 601 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac 661 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac 721 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg 781 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc 841 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 901 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 961 gtttata //