LOCUS       BBa_K175023             1022 bp    DNA     linear       16-Dec-2024
DEFINITION  PTet+Lock3c+cI+TT
ACCESSION   BBa_K175023
VERSION     BBa_K175023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..104
                     /label=BBa_J23031
     misc_feature    1..104
                     /label=BBa_K175013
     CDS             114..821
                     /label=cI lambda
     misc_feature    822..854
                     /label=LVA
     misc_feature    861..885
                     /label=Help:Barcodes
     CDS             111..885
                     /label=BBa_C0051
     stem_loop       905..948
                     /label=stem_loop
     misc_feature    894..973
                     /label=BBa_B0010
     terminator      894..973
                     /label=BBa_B0010
     stem_loop       989..1008
                     /label=T7 TE
     misc_feature    1015..1015
                     /label=stop
     polyA_site      1009..1022
                     /label=polya
     misc_feature    982..1022
                     /label=BBa_B0012
     terminator      982..1022
                     /label=BBa_B0012
     terminator      894..1022
                     /label=BBa_B0015
     misc_feature    111..1022
                     /label=BBa_K175019
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaactagaa tcacctcttg cttttgggta agacgaagag gagatactag atgagcacaa
      121 aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg
      181 aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg
      241 ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg
      301 cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag
      361 aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt
      421 accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca
      481 aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc
      541 ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg
      601 gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca
      661 gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt
      721 tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg
      781 ggaaagttat cgctagtcag tggcctgaag agacgtttgg cgctgcaaac gacgaaaact
      841 acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca
      901 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      961 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1021 ta
//