##gff-version 3
##sequence-region BBa_K175023 1 1022
BBa_K175023	.	engineered_region	111	1022	.	+	0	ID=BBa_K175019;Name=BBa_K175019
BBa_K175023	.	CDS	111	885	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K175019
BBa_K175023	.	tag	822	854	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K175023	.	CDS	114	821	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K175023	.	engineered_tag	861	885	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K175023	.	terminator	894	1022	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K175019
BBa_K175023	.	terminator	982	1022	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K175023	.	engineered_region	982	1022	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175023	.	stem_loop	989	1008	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175023	.	stop_codon	1015	1015	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175023	.	polyA_site	1009	1022	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175023	.	terminator	894	973	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K175023	.	engineered_region	894	973	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K175023	.	stem_loop	905	948	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175023	.	engineered_region	1	104	.	+	0	ID=BBa_K175013;Name=BBa_K175013
BBa_K175023	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K175013
BBa_K175023	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K175023	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K175023	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K175023	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K175023	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K175023	.	mature_transcript_region	63	104	.	+	0	ID=BBa_J23031;Name=BBa_J23031;Parent=BBa_K175013
>BBa_K175023
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctctt
gcttttgggtaagacgaagaggagatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgca
cgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatgggga
tggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaat
tctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcag
ccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacct
ttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaagg
taattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggct
gttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtc
aggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttat
cgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatag
tgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata