LOCUS       BBa_K175026             1414 bp    DNA     linear       22-Dec-2024
DEFINITION  PLacI+Key3c+TT+PTet+Lock3c+GFP+TT
ACCESSION   BBa_K175026
VERSION     BBa_K175026.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..302
                     /label=BBa_J23008
     stem_loop       322..365
                     /label=stem_loop
     misc_feature    311..390
                     /label=BBa_B0010
     terminator      311..390
                     /label=BBa_B0010
     stem_loop       406..425
                     /label=T7 TE
     misc_feature    432..432
                     /label=stop
     polyA_site      426..439
                     /label=polya
     misc_feature    399..439
                     /label=BBa_B0012
     terminator      399..439
                     /label=BBa_B0012
     misc_feature    448..466
                     /label=TetR 1
     misc_feature    448..501
                     /label=BBa_R0040
     promoter        467..472
                     /label=-35
     misc_feature    473..491
                     /label=TetR 2
     promoter        490..495
                     /label=-10
     promoter        448..501
                     /label=BBa_R0040
     misc_feature    510..551
                     /label=BBa_J23031
     CDS             558..1277
                     /label=GFP protein
     CDS             558..1277
                     /label=BBa_E0040
     stem_loop       1297..1340
                     /label=stem_loop
     misc_feature    1286..1365
                     /label=BBa_B0010
     terminator      1286..1365
                     /label=BBa_B0010
     stem_loop       1381..1400
                     /label=T7 TE
     misc_feature    1407..1407
                     /label=stop
     polyA_site      1401..1414
                     /label=polya
     misc_feature    1374..1414
                     /label=BBa_B0012
     terminator      1374..1414
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagac ccaaaagcaa gaggtgattc tagttggtgg
      241 ttaatgaaaa ttaacttact tactagaaat atctctaaaa agccagatta ttaatccggc
      301 tttactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      361 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      421 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc
      481 agtgatagag atactgagca ctactagaga actagaatca cctcttgctt ttgggtaaga
      541 cgaagaggag atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
      601 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      661 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      721 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
      781 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      841 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      901 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      961 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     1021 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     1081 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1141 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1201 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1261 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1321 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1381 ggctcacctt cgggtgggcc tttctgcgtt tata
//