LOCUS BBa_K175026 1414 bp DNA linear 22-Nov-2024 DEFINITION PLacI+Key3c+TT+PTet+Lock3c+GFP+TT ACCESSION BBa_K175026 VERSION BBa_K175026.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 209..302 /label=BBa_J23008 stem_loop 322..365 /label=stem_loop misc_feature 311..390 /label=BBa_B0010 terminator 311..390 /label=BBa_B0010 stem_loop 406..425 /label=T7 TE misc_feature 432..432 /label=stop polyA_site 426..439 /label=polya misc_feature 399..439 /label=BBa_B0012 terminator 399..439 /label=BBa_B0012 misc_feature 448..466 /label=TetR 1 misc_feature 448..501 /label=BBa_R0040 promoter 467..472 /label=-35 misc_feature 473..491 /label=TetR 2 promoter 490..495 /label=-10 promoter 448..501 /label=BBa_R0040 misc_feature 510..551 /label=BBa_J23031 CDS 558..1277 /label=GFP protein CDS 558..1277 /label=BBa_E0040 stem_loop 1297..1340 /label=stem_loop misc_feature 1286..1365 /label=BBa_B0010 terminator 1286..1365 /label=BBa_B0010 stem_loop 1381..1400 /label=T7 TE misc_feature 1407..1407 /label=stop polyA_site 1401..1414 /label=polya misc_feature 1374..1414 /label=BBa_B0012 terminator 1374..1414 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagac ccaaaagcaa gaggtgattc tagttggtgg 241 ttaatgaaaa ttaacttact tactagaaat atctctaaaa agccagatta ttaatccggc 301 tttactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 361 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 421 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc 481 agtgatagag atactgagca ctactagaga actagaatca cctcttgctt ttgggtaaga 541 cgaagaggag atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 601 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 661 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 721 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 781 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 841 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 901 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 961 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 1021 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 1081 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 1141 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 1201 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 1261 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1321 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1381 ggctcacctt cgggtgggcc tttctgcgtt tata //