LOCUS       BBa_K175033             1085 bp    DNA     linear       12-Jun-2024
DEFINITION  GFP generator with weak RBS (B0031)
ACCESSION   BBa_K175033
VERSION     BBa_K175033.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    215..218
                     /label=conserved
     RBS             209..222
                     /label=BBa_B0031
     CDS             229..948
                     /label=GFP protein
     CDS             229..948
                     /label=BBa_E0040
     stem_loop       968..1011
                     /label=stem_loop
     misc_feature    957..1036
                     /label=BBa_B0010
     terminator      957..1036
                     /label=BBa_B0010
     stem_loop       1052..1071
                     /label=T7 TE
     misc_feature    1078..1078
                     /label=stop
     polyA_site      1072..1085
                     /label=polya
     misc_feature    1045..1085
                     /label=BBa_B0012
     terminator      1045..1085
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat gcgtaaagga
      241 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      301 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      361 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      421 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      481 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      541 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      601 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      661 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
      721 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
      781 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
      841 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      901 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag
      961 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1021 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1081 ttata
//