LOCUS       BBa_K175034             1413 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive expression of GFP with weak RBS lock and inducible 
            production of key for the lock
ACCESSION   BBa_K175034
VERSION     BBa_K175034.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..294
                     /label=BBa_K175030
     stem_loop       314..357
                     /label=stem_loop
     misc_feature    303..382
                     /label=BBa_B0010
     terminator      303..382
                     /label=BBa_B0010
     stem_loop       398..417
                     /label=T7 TE
     misc_feature    424..424
                     /label=stop
     polyA_site      418..431
                     /label=polya
     misc_feature    391..431
                     /label=BBa_B0012
     terminator      391..431
                     /label=BBa_B0012
     misc_feature    440..458
                     /label=TetR 1
     misc_feature    440..493
                     /label=BBa_R0040
     promoter        459..464
                     /label=-35
     misc_feature    465..483
                     /label=TetR 2
     promoter        482..487
                     /label=-10
     promoter        440..493
                     /label=BBa_R0040
     misc_feature    537..550
                     /label=BBa_B0031
     misc_feature    502..550
                     /label=BBa_K175029
     CDS             557..1276
                     /label=GFP protein
     CDS             557..1276
                     /label=BBa_E0040
     stem_loop       1296..1339
                     /label=stem_loop
     misc_feature    1285..1364
                     /label=BBa_B0010
     terminator      1285..1364
                     /label=BBa_B0010
     stem_loop       1380..1399
                     /label=T7 TE
     misc_feature    1406..1406
                     /label=stop
     polyA_site      1400..1413
                     /label=polya
     misc_feature    1373..1413
                     /label=BBa_B0012
     terminator      1373..1413
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagac ccaaagtcct cacacaggaa acctggttaa
      241 tgaaaattaa cttaggtttc cactgtgaaa aaagccgagt tattaatccg gctttactag
      301 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      361 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      421 ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag
      481 agatactgag cactactaga ggtaggattc ctgtgtgagg actttgggta gatcactcac
      541 acaggaaacc tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
      601 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      661 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      721 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      781 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      841 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      901 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      961 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1021 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     1081 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1141 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     1201 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1261 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1321 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1381 gctcaccttc gggtgggcct ttctgcgttt ata
//