##gff-version 3 ##sequence-region BBa_K175034 1 1413 BBa_K175034 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_K175034 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_K175034 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K175034 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K175034 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_K175034 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K175034 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K175034 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K175034 . terminator 303 382 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_K175034 . stem_loop 314 357 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K175034 . engineered_region 303 382 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K175034 . mature_transcript_region 209 294 . + 0 ID=BBa_K175030;Name=BBa_K175030 BBa_K175034 . CDS 557 1276 . + 0 ID=BBa_E0040;Name=BBa_E0040 BBa_K175034 . CDS 557 1276 . + 0 ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040 BBa_K175034 . promoter 440 493 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_K175034 . promoter 459 464 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_K175034 . non_covalent_binding_site 465 483 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_K175034 . engineered_region 440 493 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_K175034 . non_covalent_binding_site 440 458 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_K175034 . promoter 482 487 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_K175034 . terminator 391 431 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_K175034 . engineered_region 391 431 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K175034 . stop_codon 424 424 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K175034 . stem_loop 398 417 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K175034 . polyA_site 418 431 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K175034 . mature_transcript_region 502 550 . + 0 ID=BBa_K175029;Name=BBa_K175029 BBa_K175034 . engineered_region 537 550 . + 0 ID=annotation2027753;Name=BBa_B0031;Parent=BBa_K175029 BBa_K175034 . terminator 1285 1364 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_K175034 . stem_loop 1296 1339 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K175034 . engineered_region 1285 1364 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K175034 . terminator 1373 1413 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_K175034 . engineered_region 1373 1413 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K175034 . stop_codon 1406 1406 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K175034 . stem_loop 1380 1399 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K175034 . polyA_site 1400 1413 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 >BBa_K175034 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagacccaaagtcctcacacaggaaacctggt taatgaaaattaacttaggtttccactgtgaaaaaagccgagttattaatccggctttactagagccaggcatcaaata aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca ctggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcag tgatagagatactgagcactactagaggtaggattcctgtgtgaggactttgggtagatcactcacacaggaaacctac tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta tacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata