LOCUS       BBa_K175035             1409 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive expression of GFP with medium RBS lock and inducible 
            production of key for the lock
ACCESSION   BBa_K175035
VERSION     BBa_K175035.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..292
                     /label=BBa_K175032
     stem_loop       312..355
                     /label=stem_loop
     misc_feature    301..380
                     /label=BBa_B0010
     terminator      301..380
                     /label=BBa_B0010
     stem_loop       396..415
                     /label=T7 TE
     misc_feature    422..422
                     /label=stop
     polyA_site      416..429
                     /label=polya
     misc_feature    389..429
                     /label=BBa_B0012
     terminator      389..429
                     /label=BBa_B0012
     misc_feature    438..456
                     /label=TetR 1
     misc_feature    438..491
                     /label=BBa_R0040
     promoter        457..462
                     /label=-35
     misc_feature    463..481
                     /label=TetR 2
     promoter        480..485
                     /label=-10
     promoter        438..491
                     /label=BBa_R0040
     misc_feature    534..546
                     /label=Medium RBS B0032
     misc_feature    500..546
                     /label=BBa_K175031
     CDS             553..1272
                     /label=GFP protein
     CDS             553..1272
                     /label=BBa_E0040
     stem_loop       1292..1335
                     /label=stem_loop
     misc_feature    1281..1360
                     /label=BBa_B0010
     terminator      1281..1360
                     /label=BBa_B0010
     stem_loop       1376..1395
                     /label=T7 TE
     misc_feature    1402..1402
                     /label=stop
     polyA_site      1396..1409
                     /label=polya
     misc_feature    1369..1409
                     /label=BBa_B0012
     terminator      1369..1409
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagac ccaaagtcct cacacaggaa agtggttaat
      241 gaaaattaac ttactttcct gactgaaaaa agccgagtta ttaatccggc tttactagag
      301 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      361 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      421 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag
      481 atactgagca ctactagagg tactatcctg tgtgaggact ttgggtagat cactcacaca
      541 ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      601 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      661 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      721 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
      781 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      841 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      901 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      961 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1021 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1081 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1141 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1201 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1261 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1321 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1381 accttcgggt gggcctttct gcgtttata
//